水库水有机污染物遗传毒性的研究

Study on the Genotoxicity of Organic Pollutants of the Water From a Reservoir

为研究水库水经国内常规给水处理工艺的各阶段出水中有机提取物对细胞DNA损伤的情况,于8月和1月取水库水作为原水,设定采集4个水样:原水(A)、经混凝沉淀后的沉后水(B)、滤后水(C)和经氯消毒的出水(D)。提取水样中的有机物配成不同浓度溶液,喂食小白鼠,取小白鼠脾脏细胞做彗星实验。结果无论在枯水期还是丰水期,水处理工艺各阶段出水对DNA都有不同程度的损伤,损伤程度顺序:A>B>D>C;丰水期水样对DNA损伤程度高于枯水期水样对DNA的损伤。结果表明,水库水中的有机物污染物具有一定的遗传毒性,常规给水处理工艺对遗传毒性有削减作用,但不能消除。加氯消毒增加了水库水的遗传毒性。

Objective to investigate the effect of the reservoir water in different periods through the stages of conventional water treatment process on cell DNA damage. The raw water was collected in August and January in reservoir water. The water treatment process is accordant to international common technique for water treatment. Four water samples were col- lected： source water（A）, settled water after the addition of coagulant（B）, filtered water after sand filter（C）, finished water disinfected with C12, （D）. Organic exttacts from water samples were configured into high concentration, medium concentra- tion and low concentration to feed female mice. Continued the experiment for five days, then killed the mice, and got lym- phocytes of the miee to do the eomet assay. Both in the dry season and wet season, water samples in different stages of the water treatment process have different degree of damage on DNA, and the order DNA damage degree： A〉B〉D〉C; Alto- gether, the DNA damage degree of the water in wet season is higher than that of the water in dry season. Reservoir water have certain genetic toxicity because of organic pollutants in the water, the conventional water treatment process on genetic toxicity has cut effect, but does not eliminate. Chlorine disinfection increased the genetic toxicity of water. The genetic tox- icity of reservoir in wet season is higher than that in dry season.