反相高效液相色谱法测定阿立哌唑血药浓度

Determineing the Serum Soncentration of Aripiprazole by RP-HPLC

目的建立测定人血清中阿立哌唑浓度的RP—HPLC法。方法以Shim—pack VP—ODSC18（150×4．6mmI．D．）为色谱柱，流动相为甲醇-乙腈-水（80：190：255），其中加5ml正丁胺调整峰型，并用醋酸将PH值调到5．5，流速为1．0ml／min，检测波长257nm，以乙醚为提取剂。结果阿立哌唑的高（800ng／ml）、中（400ng／ml）、低（200ns／ml）3个浓度的提取回收率分别为100．63％，100．38％，93．84％；分析方法的最低定量限为25ng／ml。线性范围为25—800ng／ml，回归方程为Y=290．287X-5．65288，r=0．9995（n=6）。结论该方法灵敏、准确、成本低、专一性强，可满足临床治疗过程中的阿立哌唑血药浓度测定需求。

Objective To establish a method for determining the serum concentration of aripiprazole by RP - HPLC. Methods The residues were analyzed in a Shim-pack VP- ODS C18 （150 ×4.6 mm I. D. ） with the mobile phase consisted of methanol and acetonitrile and water（80： 190：255 ）, add 5ml n - butylamine adjusting peak type, the pH to 5.5 with acetic acid, and the flow rate was 1 ml/min, the detection wave length was at 257 nm, extraction solvent was ethyl ether. Results The average recoveries for aripiprazole were 100.63%, 100.38% and 93.84% respectively. The limits of quantitation for aripiprazole was 25 ng/ml. The calibration curves for aripiprazole had good linearity, Y = 290. 287X - 5. 65288, r = 0. 9995 （ n = 6）, over the range of 25 - 800 ng/ml. Conclusion The method is sensitive, accurate,low cost, high specificity, and can meet the needs of aripiprazole blood concentration of clinical treatment in the determination of demand.