FoxM1基因表达与甲状腺乳头状癌TPC-1细胞活性及侵袭性关系的研究

Correlation of FoxM1 with the activity and invasiveness of papillary thyroid carcinoma cell TPC-1

目的 研究FoxM1基因表达水平对甲状腺乳头状癌（papillary thyroid carcinoma,PTC）细胞活性和侵袭性有无影响,探讨FoxM1与PTC疾病的相关性.方法 分别用hFoxM1-RNA干扰和硫链丝菌素处理PTC TPC-1细胞,观察处理后的癌细胞与未经处理的癌细胞在细胞活性、侵袭能力、迁移能力方面的变化,并使用Real-time PCR检测各组细胞中FoxM1基因的表达水平,观察硫链丝菌素对FoxM1基因表达水平的影响.结果 在TPC-1细胞中,使用hFoxM 1-RNA干扰和硫链丝菌素处理均能使细胞活性明显下降（P＜0.05）,用2种方法处理后,细胞侵袭能力分别降低了约29.2％及38.63％,细胞迁移能力分别降低了约46.0％及47.5％,且PCR结果表明,hFoxM1-RNA干扰和硫链丝菌素处理均明显抑制TPC-1细胞中FoxM1基因mRNA的表达,分别抑制55％及38％.结论 FoxM1能促进PTC细胞的侵袭转移,其作为潜在的肿瘤标志物值得关注,而硫链丝菌素能抑制肿瘤细胞中FoxM1的表达.

Objective To study correlation of FoxM1 expression level with the activity and invasiveness in papillary thyroid carcinoma （PTC）cell line TPC-1.Methods TPC-1 cells were treated by hFoxM1-RNA interference or thiostrepton.The changes of cell activity,invasiveness and migration of treated TPC-1 cells were observed.Real-time PCR was used to measured the expression of FoxM1 in TPC-1 cells or thiostrepton treated TPC-1 cells.Results In TPC-1 cells,either hFoxM1-RNA interference or thiostrepton significantly inhibited the cell activity.The cell invasiveness decreased 29.2％ or 38.63％ and migration decreased 46.0％ or 47.5％ after the treatment of hFoxM1-RNA interference or thiostrepton.Treatment of hFoxM1-RNA interference or thiostrepton significantly inhibited mRNA level of FoxM1 in TPC-1 cells,the inbition was 55％ or 38％.Conclusions FoxM1 can promote the invasion and metastasis of PTC cells.It suggests that FoxM1 can be used as a potential tumor marker,and thiostrepton can inhibit FoxM1 expression in tumor cells.