原始红松林与人工林土壤微生物多样性分析

Study on the microbial diversity of primary Korean pine forests and artificial forests

以土壤微生物为研究对象，分析了小兴安岭凉水国家自然保护区内原始红松林及人工林土壤微生物多样性。结果表明：AWCD值随土深与季节变化表现为，在表层与0～10 cm层土中以红松林和紫椴林最高；在10～20 cm层土中以兴安落叶松林在春、夏、秋3季最高，冬季以椴树林最高。各土层的微生物多样性指数均以椴树林最高，且显著高于人工林，综合土壤微生物群落碳源利用类型主成分分析结果得知：原始红松林土壤微生物多样性特征比较明显。

By the use of BIOLOG microplate method , the soil microbial diversity characteristics of primary Korean pine forests and man-made forest in Liangshui National Nature Reserve , Lesser khingan were studied .The results showed that the soil microbial AWCD values of the forest-type varied with soil depth and seasons：in the surface and 0-10 cm layer of soil , it was the highest in Korean pine forest and Tilia amurensis forest; in the 10-20 cm layer of soil, it was the highest in Xing＆#39;an larch forest in spring, summer and autumn, while it was the highest in Tilia amu-rensis forest in winter.The soil microbial diversity indexes of all soil layers were the highest in Tilia amurensis forest, and were significantly higher than those of man-made forest.So we concluded that the soil microbial diversity of pri-mary Korean pine forest was richer than that of the others .