内蒙古中西部地区汉族、蒙古族ApoB基因遗传多态性

ApoB Gene Genetic Polymorphism of Han Nationality and Mongolian Nationality in Midwest Area of Inner Mongolia

目的研究内蒙古中西部地区汉族、蒙古族ApoB基因遗传多态性。方法选取内蒙古中西部地区汉族、蒙古族无关个体,采用聚合酶链反应-限制性片段长度多态性技术,判断样本中是否含有ApoB基因中的稀有等位基因:XbaⅠ(X+)和EcoRⅠ(E-),并计算其基因型频率、等位基因频率及相关的群体遗传学参数。结果内蒙古汉族群体中稀有等位基因XbaⅠ(X+)和EcoRⅠ(E-)频率分别为2%和4.6%,而在蒙古族群体中没有检测出此两种稀有等位基因。结论 ApoB基因XbaⅠ和EcoRⅠ位点的等位基因频率分布在不同种族中差异较大,具有种族鉴定的应用可能。

Objective To study ApoB gene genetic polymorphism of Han nationality and Mongolian na tionality in midwest area of Inner Mongolia. Methods Some unrelated individuals of Han nationality and Mongolian nationality in midwest area of Inner Mongolia were selected. Polymerase chain reaction-restric tion fragment length polymorphism technology was used to check the presence of Xba （X-） and EcoR I （E-） sites of rare alleles. The genotype frequency, allelic frequency and population genetics parameters were calculated. Results The frequencies of Xba I （X＋） and EcoR I （E-） rare alleles were 2% and 4.6% in Han population. There was no Xba [ （X＋） or EcoR [ （E-） rare alleles found in Mongolian nationality. Conclusion The allelic frequencies of ApoB gene Xba I and EcoR I sites are very different in different races. These sites may be used in identification of ethnicity.