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伪狂犬病毒单克隆抗体的制备和鉴定 被引量：3

Preparation and Characterization of Monoclonal Antibody against Pseudorabies Virus
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摘要 以伪狂犬病毒(PRV)作为免疫原,免疫BALB/c小鼠,融合之后经间接ELISA、IFA和IHC试验进行杂交瘤筛选,共获得2株能分泌针对PRV单克隆抗体(简称单抗)的杂交瘤细胞株,2株猪伪狂犬病毒单抗与猪细小病毒、猪繁殖与呼吸综合征病毒、猪圆环病毒2型、猪瘟病毒均无交叉反应,IFA结果显示单抗能与接种于猪睾丸细胞(ST)的PRV发生特异性反应,IHC结果显示单抗能用感染了PRV的猪神经组织发生反应,证实抗PRV单克隆抗体具有良好的特异性和敏感性,此结论为猪伪狂犬病毒抗原表位分析及相关抗原抗体诊断试剂盒的研制奠定了基础。 By taking PRV as immunogen, BALB/c mice as model and using indirect ELISA, IHC and IFA for hybridoma screening, we found out that 2 hybridoma cell line strains could secrete PRRSV monoclonal antibody （hereafter referred to as McAb）. These two strains showed single resistance to porcine parvovirus, porcine pseudo rabies virus and porcine wreath virus type 2 also. IHC and IFA results also supported the single resistance to all above viruses, and the vaccination in ST cells infected with PRV showed specific reaction, which proved that these monoclonal antibody had good specificity and sensitivity. Preparatiom of PRV monoclonal antibody could provide valuable tools for further development of antigen epitope analysis and specific diagnosis reagent.
作者 李建 曹娟 廖园园 刘洁 朱微 秦伟 秦红刚 漆世华 谢红玲
机构地区 武汉中博生物股份有限公司
出处 《中国兽药杂志》 2014年第3期6-9,共4页 Chinese Journal of Veterinary Drug
关键词 伪狂犬病毒 单克隆抗体 特异性 敏感性 pseudorabies virus monoclonal antibody specificity sensitivity
分类号 S852.655 [农业科学—基础兽医学]
  • 相关文献

参考文献9

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共引文献16

同被引文献33

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引证文献3

二级引证文献9

中国兽药杂志
2014年 第3期

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