摘要
目的 :观察复方血栓通胶囊对局灶性脑缺血再灌注损伤后大鼠脑组织Bcl-2和Bax表达的影响。方法:采用线栓法制作局灶性脑缺血再灌注大鼠模型,将36只成年SD大鼠随机分为假手术组、缺血再灌注组和复方血栓通胶囊治疗组,每组12只,各组分别于再灌注后24 h处死取出脑脑织,采用免疫组织化学法和半定量RT-PCR法检测各组缺血区脑组织Bcl-2和Bax表达的变化。结果 :与缺血再灌注组相比,复方血栓通胶囊治疗组脑组织中Bcl-2的表达水平明显增高,Bax的表达水平明显下调。结论 :复方血栓通胶囊对脑缺血再灌注大鼠的保护作用,其作用机制可能与抑制细胞凋亡有关。
Objective： To investigate the effects of compound Xueshuantong capsule on the expressions of Bcl-2 and Bax in the brain after focal cerebral ischemia-reperfnsion injury in rats. Methods ： 36 male aduh SD rats were randomly divided into 3 groups： sham-operation group, isehemia-reperfusion group and compound Xueshuantong treatment group. The rats were killed to detect the expressions of Bel-2 and Bax in ischemic region by immunohistochemlstry and RT-PCR at 24 h. Resuhs： Compared with the ischemia-reperfnsion group, the expression of Bcl-2 was increased while the expression of Bax was decreased significantly in compound Xueshuantong treatment group. Conclusion ： The compound Xueshuantong capsule can lighten the neurons injury after focal cerebral ischemia-reperfusion injury in rats partly by increasing the expression of Bel-2 and deereasing that of Bax.
出处
《辽宁中医药大学学报》
CAS
2014年第3期20-22,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
泉州市科技计划项目(2012Z78)
