铜绿假单胞菌对致病真菌的抑制作用

The inhibiting effect of pseudomonas aeruginosa to the growth of pathogenic fungi

目的观察临床分离的24株铜绿假单胞菌(PA)对致病性真菌(如白色念珠菌、热带念珠菌、光滑念珠菌、近平滑念珠菌、克柔念珠菌、葡萄牙念珠菌等)的抑制作用。方法收集临床上24株PA,通过革兰染色、氧化酶试验及API 20NE(法国梅里埃公司产品)进行鉴定;通过纸片法、十字交叉划线法及共培养检测PA对致病性真菌的抑制作用。十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对有抑制作用的PA和无抑制作用的PA分泌蛋白进行电泳。结果 24株PA中部分菌株对常见的致病性真菌有显著抑制作用,而部分PA菌株有一定抑制作用,另外一些完全没有抑制作用;共孵育试验显示PA可能是通过抑制真菌的菌丝生长而产生抑制作用。SDS-PAGE显示有抑制作用的PA和无抑制作用的PA分泌物蛋白间存在明显差异。结论PA对常见的致病真菌具有抑制作用,推测可能与抑制真菌的菌丝生长及分泌抑制真菌生长的各种蛋白类物质有关。

Objective To explore the inhibitory effect of 24 pseudomonas aeruginosa（PA） on pathogenic fungi,such as candida albicans,candida tropicalis,candida glabrata,candida parapsilosis,candida krusei,mucous spore bacterium（MSB） etc. Methods 24 PA isolates were collected from clinical specimens and identified by Gramrs stain,oxidase production and the API 2ONE system（bi- oMerieux,France）. Cross-streaking method and sterilizing filter paper-disk method and co-cultured method were applied to observe the inhibitory effect of PA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） analyzed the difference of bacte- rial proteins of PA. Results The results showed that some strains of 24 PA had strong inhibitory effect against pathogenic fungi, some strain had partial effect and others had no effect. Co-cultured test showed that PA could inhibit the growth of fungal hyphae. SDS-PAGE displayed the significant difference in secretive proteins between the PA strains which had strong effect and no effect. Conclusion PA have inhibitory effect upon common pathogenic fungi and and this might be related to inhibit fungal hyphae forma- tion, various protein secretion and inhibit the growth of fungi.