荧光定量PCR法和基因芯片分型法检测人乳头瘤病毒的敏感度比较研究

Comparative research on fluorescent quantitation PCR method and gene chips typing method in testing sensitivity of human papilloma virus

目的比较荧光定量聚合酶链反应法(简称荧光定量法)与基因芯片分型法(简称基因芯片法)在检测人乳头瘤病毒(HPV)中的敏感度。方法选取2010年2月至2013年2月来该院妇科门诊就诊的患者305例,收集其宫颈脱落细胞,分别用荧光定量法和基因芯片法检测HPV,对结果不一致的病例采用测序法进行确证。305例病例均进行液基细胞学检测。结果荧光定量法检测HPV敏感度为33.8%,基因芯片法检测HPV敏感度为37.6%,两种方法具有较高的一致性,HPV感染率随宫颈脱落细胞异型性增加而增加。结论荧光定量法和基因芯片法检测HPV具有较高的一致性,其中基因芯片法敏感度较高。宫颈细胞学改变的严重程度与HPV感染情况呈正相关。

Objective To compare the sensitivity of the fluorescent quantitation PCR method （fluorescence quantitative method for short）and the gene chips typing method （gene chips method for short）in testing human papilloma virus（HPV） .Methods 305 outpatients in the gynecological clinic of the hospital from February 2010 to February 2013 were selected as the research subject . Then the cervical exfoliated cells were collected and detected HPV by the fluorescence quantitative method and the gene chips meth-od respectively .Those cases with inconsistent results were confirmed by the PCR sequencing method .305 cases were performed the liquid based cytology test .Results The sensitivity of the fluorescence quantitative method in detecting HPV was 33 .8% and which of the gene chips method was 37 .6% .The two methods had a higher conformance .The HPV infection rate was increased with the increase of cervical exfoliated cell atypia .Conclusion The fluorescence quantitative method and the gene chips method have a higher conformance .The sensitivity of the gene chips method is higher .The severity degree of cervical cytological change is positively cor-related with the HPV infection .