VitC、KCl及止血敏对3种交叉配血方法的影响探讨

Investigation on influences of vitamin C,KCL and dicynone on 3 kinds of blood cross matching method

目的了解药物维生素C(VitC)、氯化钾(KCl)及止血敏对盐水介质、微柱凝胶配血法的影响,并与凝聚胺配血法对比。方法分别在含与不含有特异性抗体两种条件下,加入KCI注射液、酚磺乙胺(止血敏)注射液、VitC注射液、生理盐水,与相应红细胞进行免疫学反应。观察3种药物对不同介质配血方法的影响。结果低浓度的VitC、KCl不影响凝聚胺介质配血,止血敏令试验产生假凝集;高浓度的3种药物可导致试验失败。盐水介质法检测敏感性略低,VitC、KCl对试验的影响不明显,而KCl组随着抗体被稀释,凝集强度减弱甚至于消失。结论 3种药物对微柱凝胶配血法均有影响,尤其受KCl影响较大。

Objective To comprehend the influences of vitamin C,KCL and dicynone on blood cross matching of saline and mi- erocolumn gel and to compare them with polybrene crossing matching. Methods Under the conditions with and without the specific antibody, K CI injection, d icynone injection, vitamin C injection and normal saline were added for conducting the immunological reac- tion with corresponding RBC. The influences of 3 kinds of drug on different medium cross matching methods of different mediums. Results The low concentration of vitamin C and KCL does not affect polybrene cross matching,dieynone makes the experiment to generate pseudoagglutination;the high concentration of these 3 kinds of drug can cause the test to fail. The sensitivity of the saline medium method is slightly lower,the influence of vitamin C and KCL on the test is inapparent,while in the KCL method,with the dilution of antibody,the agglutination intensity is weakened and even disappeared. Conclusion 3 kinds of drug all have influences on the mierocolumn gel cross matching method,especially KCl.