^(133)Cs胁迫对转IrrE油菜生理活性的影响

Effect of ^(133)Cs Stress on Physiological Characteristics of IrrE-transgenic Rape Seedlings

以转IrrE基因油菜为材料,以非转基因油菜为对照,砂培条件下研究了油菜对Cs的积累吸收情况和Cs胁迫对油菜SOD、POD酶活性、MDA含量及游离脯氨酸含量的影响.结果表明:随着处理浓度的增大,转基因油菜和非转基因油菜对Cs的吸收量都增大,二者根部的吸收量都大于其地上部分;转基因油菜根部的积累量大于非转基因油菜根部的量,而地上部分积累量小于非转基因油菜的含量;在不同Cs浓度的胁迫下,转基因和非转基因叶片的SOD和POD活性均呈现先升后降的趋势,4种Cs浓度处理下转基因油菜活性都高于非转基因油菜;转基因和非转基因油菜叶片的MDA含量均呈上升的趋势,但转基因油菜体内的MDA含量都低于非转基因油菜;4种处理下油菜叶片脯氨酸含量也表现出上升趋势,而转基因油菜产生的游离脯氨酸高于非转基因产生的.以上结果说明在油菜受到Cs胁迫时,转IrrE基因油菜比非转基因油菜更具有Cs胁迫耐受性.

In this paper, Irre -transgenic rape seedlings under quartz sand culture was employed to investigate the Cs uptake and effects of Cs stress on MDA content, Pro content, SOD and POD activity. The results showed that the uptake of Cs in transgenic and non - transgenic rape increased with the increase of concentration and the Cs content in their roots more than that of their over - ground parts ; the Cs uptake in transgenic rape roots was more than that in non - transgenic rape roots, but the Cs uptake in the over - ground part of transgenic rape was less than that of non - transgenic rape. The activities of SOD and POD in transgenic and non - transgenic rape were increased firstly and then decreased with Cs concentration increase. The SOD and POD activity of transgenic rape seedlings was higher than that of the non - transgenic under four different Cs concentration treatments. The MDA content in transgenic and non -transgenic rape leaves was increased, but the MDA content in transgenic rape seedlings was lower than that in non -transgenic rape under all the treatments. The proline content in rape leaves under four treatments also increased, while transgenic rape produces proline content more than non - transgenic rape. These results from above suggested that IrrE -transgenic rape has better ability to tolerance Cs stress when rape is in Cs stress.