扛板归及苦菜黄酮类物质超声波提取工艺优化及其抗氧化活性

Ultrasonic extraction technology optimization and antioxidant activities of flavonoids from Polygonum perfoliatum L. and Sonchus oleraceus L.

研究了扛板归黄酮超声波提取条件及其抗氧化作用,并与苦菜黄酮的含量及其抗氧化作用进行了对比。采用单因素和正交实验得出扛板归黄酮的最佳提取条件为料液比1∶50,体积分数65%乙醇,超声波处理时间50min,在此条件下扛板归黄酮的最高得率为70.77mg/g,苦菜黄酮得率为18.54mg/g。两种黄酮类物质在不同体系中对·OH的清除作用不同,水杨酸体系苦菜黄酮对·OH的清除效果强于扛板归黄酮,分别是芦丁和VC的7.9倍和1.35倍,而在邻二氮菲体系扛板归黄酮对·OH的清除效果高于苦菜黄酮,大约是芦丁和VC的2.5倍。

The optimal ultrasonic extraction technology and antioxidant activities of flavonoids from Polygonum perfoliatum L.and Sonchus oleraceus L.were studied and compared.The optimal flavonoids extraction conditions were acquired with single factor experiment and orthogonal experiment.The optimal extraction condition was solid-liquid ratio 1∶50,ethanol 65％vol,and ultrasonic extraction time 50min.Under this condition,the flavonoids yield of P.perfoliatum and S.oleraceus were 70.77mg/g and 18.54mg/g,respectively.The inhibition rate of two kinds of flavonoides on · OH were different in different system,the scavenging activity of flavonoids from S.oleraceus was higher than flavoniods from P.perfoliatum,and they were 7.9 and 1.35 times than that of rutin and Vc in salicylic acid system,respectively.While in phenanthroline system,the inhibition rate of flavonoides from P.perfoliatum was higher than flavoniods from S.oleraceus,and the inhibition activity on ·OH were approximately 2.5 times than rutin and VC.