广谱抗生素对大鼠肠道菌群多样性影响的16S rDNA基因测序研究

THE EFFECT OF BROAD-SPECTRUM ANTIBIOTIC ON GUT MICROBIOTA IN RATS BASED ON 16S rDNA SEQUENCING

目的探讨基于16SrDNA基因测序技术的广谱抗生素对大鼠肠道菌群多样性的影响。方法将24只Wistar大鼠随机分为一次应用抗生素组、多次应用抗生素组和对照组,每组8只。取用药前和用药结束后大鼠粪便做16SrDNA基因测序分析,比较各组大鼠用药前后肠道菌群多样性的变化。结果一次及多次应用抗生素组大鼠的肠道菌群种类多样性指数Chao1、ACE、Shannon用药后较用药前降低,Simpson指数用药后较用药前升高,差异均有统计学意义（t=5.3~12.7,P〈0.05）。多次应用抗生素组大鼠肠道菌群用药前后的变化与一次应用抗生素组相比,多样性指数Chao1、ACE、Shannon降低幅度更明显,Simpson指数升高幅度也更明显（t=4.1~5.7,P〈0.05）。对照组大鼠肠道菌群多样性指数Chao1、ACE、Shannon及Simpson用药前后变化均无统计学意义（P〉0.05）。结论广谱抗生素可显著降低大鼠肠道菌群多样性,且用药时间越长,其降低幅度越明显。

Objective To observe the effect of broad-spectrum antibiotic on gut microbiota diversity of rat based on 16S rDNA sequencing Methods Twenty-four rats were evenly randomized to three groups as：single-antibiotic,multiple-antibiotic and control group.Rat excrement was collected（before and upon completion of medication）for 16SrDNA sequencing,the diversity index of intestinal tract microbiota was compared. Results After antibiotic treatment,Chao1,ACE and Shannon index decreased significantly,whereas Simpson index increased,as compared with before medication,the difference was significant（t= 5.3-12.7,P0.05）.Compared with single-antibiotic group,the variation of diversity indexes in multiple-antibiotic group was more significant（t=4.1-5.7,P0.05）.Diversity indexes did not vary significantly in the control group（P0.05）. ConclusionBroad-spectrum antibiotic can dramatically decrease intestinal microbiota diversity in rats,the longer the antibiotic to be used, the more the microbiota diversity decrease will be.