中药复方清肠汤对大鼠实验性结肠炎的疗效观察

Effects of traditional Chinese medicine compound Qingchangtang on experimental colitis of rats

[目的]观察中药复方清肠汤对实验性结肠炎大鼠的治疗效果。[方法]将40只SD大鼠随机分为空白对照组(A组)、模型组(B组)、西药对照组(C组)、中药常规剂量组(D组)、中药大剂量组(E组)和湿热组(F组),采用乙酸灌肠加葡聚糖硫酸钠自由饮用的方法制作大鼠实验性结肠炎模型,F组再给予高糖高脂饮食喂养并放置高温高湿环境处理以模拟大肠湿热证形成条件,造模同时D、E、F组给予清肠汤灌胃,C组给予柳氮磺嘧啶(SASP)混悬液灌胃,每日2次,治疗7d后再次以乙酸灌肠,各组均连续灌胃14d,治疗前后每日观察记录大鼠体质量、饮食及活动情况,重点观察粪便的性状改变,造模前后检测肛温,对死亡大鼠进行解剖并观察肠道病理改变。治疗结束后对各组大鼠疾病活动指数(DAI)、肠黏膜损伤评分及组织病理学评分(HI)进行统计学评价和分析。[结果]与A组相比,造模后各组大鼠DAI评分、肠黏膜损伤评分和HI评分明显上升(P<0.05);给药后,D组和F组的DAI评分有明显下降(P<0.05),各组肠黏膜损伤评分和HI评分明显降低(P<0.05),F组肠道HI评分明显低于D组(P<0.05)。[结论]清肠汤能改善实验性结肠炎模型大鼠的症状,并且与SASP一样均能使模型大鼠的肠黏膜损伤减轻。而清肠汤对湿热证模型组大鼠肠道病理损伤的改善优于非湿热证模型组,体现了中医辨证论治的优势。

[Objective] To observe Qingchangtang on group （group A）, experimental colitis model group （group the therapeutic effec of rats. [Methods] For B）, western medicine t of traditional ty SD rats were Chinese randoml treatment group （group medicine compound y divided into control C）, Chinese medicineroutine dose group （group D）, Chinese medicine high-dose group （group E） and damp-heat syndrome group （group F）. Colitis rat model was made by clystering with acetic acid and free drinking with dextran sodium sulfate. Group F was fed with high sugar and high fat diet and placed in high temperature and high humidity environment to make large intestine damp heat syndrome models. At the same time, group D, E and F were given Qingchangtang by gavage, group C given Salazosulfadimidine （SASP）, twice a day. Af- ter the treatment for seven days, the rats were clystered with acetic acid once again. Each group was given the treatment for continuous 14 days. Before and during the treatment, body weight, food-intake and ac- tivity were recorded daily, especially the stool characteristics. The rats anal temperature were tested before and after making models. The dead rats were dissected and the intestinal pathological changes were ob- served. After the treatment, disease activity index （DAD, intestinal mucosal damage rating and histopa- thology score （HI） were evaluated and analysed. [Results] Compared with group A, group B showed in- creased DA1 scores,intestinal mucosal damage scores and HI scores （P〈0.05）. After the treatment,DAI score in the group D and F decreased obviously （P〈0.05）, the intestinal mucosal damage rating and HI score also decreased significantly （P〈0.05）. Intestinal HI score in the group F was significantly lower than that of the group D （P〈0.05）. [Conelusion]Qingchangtang improved symptoms of the experimental colitis model rats and reduced intestinal mucosal damage with the same effect as SASP. Qingchangtang bad better effect on pathological damage in the treatment of the damp-heat syndrome model than the non damp- heat syndrome model, which reflected the advantage of TCM differentiation and treatment according to dif- ferent syndromes.