HPLC法测定三酸散中苯甲酸和水杨酸的含量

Determination of Benzoin Acid and Salicylic Acid in Tri-Acid Powder by HPLC

目的研究三酸散中苯甲酸和水杨酸的HPLC测定方法。方法色谱柱：C-18；紫外检测器波长：275nm；流速：1ml·min-1。流动相组成：甲醇-0．1％磷酸（50：50）。结果苯甲酸和水杨酸的线性范围分别为0．0578～0．4049mg·ml-1和0．1152～0．8066mg·ml-1回收率分别为100．0％和99．5％，RSD分别为0．97％和0．50％。结论本方法同时测定苯甲酸和水杨酸具有简便、准确和重复性好的特点，可用于三酸散的质量控制。

Objective To establish an HPLC method for determination of benzoin acid and salicylic acid in tri-acides powder. Methods An Agilent Eclipse XDB C1s（4.6 mm × 150 mm,5 μm） was used by HPLC. The de- tection wavelength was 275 nm, the flow rate was 1 ml·min- 1, and the mobile phase was methanol-0.1% phosphor- ic acid（50： 50）. Results The linear range of benzoin acid and salicylic acid was 0.0578 -0. 4049 mg ·ml- 1 and 0. 1152 -0. 8066 mg ·m1-1 respectively. The recovery rate was 100.0% and 99.5% , respectively. Conclusion This method is simple and accurate in simultaneous determination of these two components, which can be used to control the quality of tri-acid powder.