摘要
目的建立HPLC法同时测定木香健胃颗粒中木香烃内酯和去氢木香内酯含量,为质量控制提供数据支持。方法采用c。。色谱柱(150mm×4.6mm,5μm);流动相为乙腈一水体系,流速1.0ml·min-1,柱温30℃,检测波长225nm。结果指标成分木香烃内酯和去氢木香内酯进样量分别在0.508-5.588μg、0.336~3.696μg范围内线性关系良好(R2≥0.9996),加样回收率分别为101.26%、101.87%,RSD分别为0.98%、0.54%。结论本方法简便可靠,重复性好,可反映木香健胃颗粒质量情况,适用于该制剂的质量控制。
Objective To establish an HPLC method for simultaneous determination of costunolide and de- hydrocostus lactone in Muxiang Jianwei granules. Methods C ls column( 150 mm×4.6 ram,5 μm) was used. The mobile phase consisted of 0. 1% phosphoric acid solution ( A ) and acetonitrile ( B ). The flow rate was 1. 0 ml .rain-l, the temperature of the column was 30 ℃, and the detection wavelength was set at 225 nm. Results There was a good linear relationship within the range of 0. 508 - 5. 588 μg for costunolide and 0. 336 - 3. 696 μg for dehydroeostus lactone ( R2 ≥0. 9996). The average recovery was 101.26% and 101.87% respectively, with a respective RSD of 0.98% and 0.54%. Conclusion This method is simple, accurate, reliable, repeatable and can be used to control the quality of Muxiang Jianwei granules.
出处
《解放军药学学报》
CAS
2014年第1期63-65,共3页
Pharmaceutical Journal of Chinese People's Liberation Army