HPLC法测定康肾口服液中人参皂苷的含量

HPLC method for determining ginsenoside content in Kangshen oral liquid

[目的]研究康肾口服液用高效液相色谱法（HPLC）测定其人参皂苷的含量。【方法】以十八烷基硅烷键合硅胶为填充剂，以乙腈-水（18：82）为流动相，检测波长为203nm。理论塔板数按人参皂苷Rgl峰计算应不低于6000。【结果】人参皂苷Rgl浓度在0．053-0．265g／L范围内，人参皂苷Re浓度在0．037-0．185g/L范围内，峰面积与浓度线性关系良好，平均回收率为99．12％。【结论】HPLC法测定康肾口服液中人参皂苷的含量，操作简单、重复性好，可有效控制康肾口服液的质量标准。

[Objective] To study the content of the ginsenoside in Kangshen oral liquid by HPLC method. [Methods] Take eighteen alkyl silane bonded silica as filler, water solution with acetonitrile （82：18） as mobile phase; the detection wavelength was 203 nm. Number of theoretical plates by ginsenoside Rgl peak calculation should not be less than 6000. [Results] The ginsenoside Rgl concentration in the range of 0.053~0.265 g/L, ginseng saponin Re concentration in the range of 0.037~0.185 g/L, the peak area and the concentration had good linear relationship. The average recovery rate was 99.12%. [Conclusion] The HPLC method for the determination of ginsenoside content in Kangshen oral liquid has a feature of simple operation, good reproducibility. It can effectively control the quality standard of Kangshen oral liquid.