摘要
[目的]研究康肾口服液用高效液相色谱法(HPLC)测定其人参皂苷的含量。【方法】以十八烷基硅烷键合硅胶为填充剂,以乙腈-水(18:82)为流动相,检测波长为203nm。理论塔板数按人参皂苷Rgl峰计算应不低于6000。【结果】人参皂苷Rgl浓度在0.053-0.265g/L范围内,人参皂苷Re浓度在0.037-0.185g/L范围内,峰面积与浓度线性关系良好,平均回收率为99.12%。【结论】HPLC法测定康肾口服液中人参皂苷的含量,操作简单、重复性好,可有效控制康肾口服液的质量标准。
[Objective] To study the content of the ginsenoside in Kangshen oral liquid by HPLC method. [Methods] Take eighteen alkyl silane bonded silica as filler, water solution with acetonitrile (82:18) as mobile phase; the detection wavelength was 203 nm. Number of theoretical plates by ginsenoside Rgl peak calculation should not be less than 6000. [Results] The ginsenoside Rgl concentration in the range of 0.053~0.265 g/L, ginseng saponin Re concentration in the range of 0.037~0.185 g/L, the peak area and the concentration had good linear relationship. The average recovery rate was 99.12%. [Conclusion] The HPLC method for the determination of ginsenoside content in Kangshen oral liquid has a feature of simple operation, good reproducibility. It can effectively control the quality standard of Kangshen oral liquid.
出处
《天津中医药》
CAS
2014年第3期181-183,共3页
Tianjin Journal of Traditional Chinese Medicine
基金
河南省科学技术厅2009年科技发展计划(092102 310202)