白藜芦醇对D-半乳糖致衰老小鼠学习记忆能力和脑组织抗氧化能力的影响

Effects of Resveratrol on Learning,Memory and Brain Antioxidant Abilities in D-Galactose-Induced Aging Mice

探讨白藜芦醇对D-半乳糖致衰老小鼠脑组织抗氧化与学习记忆的影响,揭示白藜芦醇延缓脑衰老的作用。将50只雄性ICR鼠随机分为正常对照组,模型组,白藜芦醇低、中、高剂量组。模型组及白藜芦醇治疗组连续8周于小鼠颈背部皮下注射D-半乳糖120 mg/(kg·d),给予不同剂量白藜芦醇(25、50、100 mg/(kg·d))灌胃;正常对照组注射、灌胃等量生理盐水。采用Morris水迷宫检测各组小鼠的学习记忆能力,并测定各组小鼠脑组织超氧化物歧化酶(superoxide dismutase,SOD)、总抗氧化能力(total antioxidant capacity,T-AOC)、丙二醛(malondialdehyde,MDA)的含量。白藜芦醇能够显著缩短D-半乳糖致衰老小鼠Morris水迷宫中找到隐藏平台时间(P<0.01),提高游泳速度(P<0.01)和穿越目标象限的次数(P<0.01);提高衰老模型小鼠脑组织SOD(P<0.01)、T-AOC活性(P<0.05),降低MDA含量(P<0.01)。白藜芦醇能够改善D-半乳糖致衰老模型小鼠的学习记忆能力,提高脑组织的抗氧化能力,具有一定延缓脑衰老的作用。

Objective： To investigate the effects of resveratrol （Res） on learning, memory and brain antioxidant capacities in D-galactose-induced aging mice. Methods： Fifty ICR mice were randomly divided into five groups, designated as normal control, model, resveratrol low-dose, moderate-dose and high-dose groups. The normal control group was given normal saline by gavage, and all other groups were given D-galactose solution by neck back subcutaneous injection at the daily dose of 120 mg/（kg-d） for 8 consecutive weeks. At the same time, Res-treated mice were administered Res by gavage at the daily doses of 25, 50 and 100 mg/（kg.d） body weight per day, respectively. The learning and memory abilities of mice were determined by Morris water maze test. Superoxide dismutase （SOD）, total antioxidant capacity （T-AOC） and malondialdehyde （MDA） in the brain of mice were determined at the end of 12 h of fasting after the last administration. Results： Res could reduce the time mice spent on finding platform （P 〈 0.01）, improve the swimming speed （P 〈 0.01 ） and the number of times mice crossed the platform （P 〈 0.01） in Morris water maze test, increase SOD （P 〈 0.01） and T-AOC activities （P 〈 0.05） and decrease the content of MDA （P 〈 0.01） in brain tissue. Conclusion： Res can ameliorate the degeneration of learning and memory abilities in D-galactose-induced aging mice, improve brain antioxidant abilities and thus delay brain aging.