HPLC法测定柘木中槲皮素、染料木素和山柰素的研究

Determination of quercetin,genistein and kaempferol in Cudrania tricuspidata

目的：采用高效液相色谱法同时测定柘木中3个黄酮类成分（槲皮素、染料木素和山柰素）的含量。方法：采用ApolloC18色谱柱（4．6mm×250mm，5μm），以1％醋酸一甲醇为流动相，梯度洗脱（0～28min，65％A→40％A）；流速：1mL·min^-1；柱温：30℃；检测波长：260nm。结果：槲皮素、染料木素和山柰素进样量分别在0．159～1．59wg（r=0．9997）、0．046—0．46μg（r=0．9995）和0．048～0．48μg（r=0．9998）范围内线性关系良好；平均回收率（n=6）分别为98．2％（RSD=2．6％）、100．6％（RSD=2．9％）和100．4％（RSD=2．7％）。结论：本法为该药材质量控制研究提供了参考。

Objective： To develop a method for determining 3 flavonoid constituents （quercetin, genistein and kaempferol） in Cudrauia tricuspidata. Methods：The analysis was conducted on an Apollo C18column （4. 6 mm × 250 mm, 5 μm） eluted with 1% acetic acid and methanol as mobile phases in gradient mode （0 -28 min ,65 % A→40% A）. The flow rate was 1 mL · min-1 and the detection wavelength was set at 260 nm. The column temperature was 30 ℃. Results：The linear concentration ranges of quercetin, genistein and kaempferol were 0. 159 - 1.59 p,g （r =0. 9997） ,0. 046 -0. 46 μg （r =0. 9995） and 0. 048 -0. 48 p,g （r =0. 9998） ,respectively;The average re- coveries （ n = 6） were 98.2% （ RSD = 2. 6% ）, 100. 6% （ RSD = 2.9% ） and 100. 4% （ RSD = 2.7% ）, respec- tively. Conclusion：The method is simple, accurate and sensitive, and can be used for the quality control of Cudra- nia tricuspidata as a reference.