利用啤酒废酵母自溶制备橘小实蝇引诱剂的研究

Study on Attractant Produced by Autolysis of Waste Brewer's Yeast to Bactrocera dorsalis( Hendel)

对利用啤酒废酵母自身所含有的蛋白酶自溶酶解制备橘小实蝇引诱剂进行了深入的研究.结果表明,啤酒酵母悬浮液质量浓度为100％的沉淀酵母泥时,物理性状及蛋白质质量浓度为最佳;自溶促进剂食盐的质量浓度为3％时,酵母的风味及蛋白质质量浓度最佳;pH值为8.5 ～9.0,自溶温度为50℃以及自溶时间为44h时,蛋白质质量浓度和引诱率分别为35.65 ～ 36.16 mg/mL,60％～63.3％,109.5 mg/mL,50％和51.06 mg/mL,66.7％;在这种条件下获得的引诱剂对橘小实蝇的引诱效果最佳.

The effects of attractants, which were produced by the waste of brewer yeast that digested by the autolysis protease, on Bactrocera dorsalis （Hendel） was studied. The results showed that 100% mass concentration of yeast, which was precipitated from the suspension liquid of beer yeast, its physical char- acteristics and protein mass concentration were the best. When autolysed in the condition of 3% acceler- ant （salt）, the yeast flavor and protein mass concentration were the best. When pH 8.5 - 9. 0, autol- ysed in 50℃ or for 44 h, its protein mass concentrations and attraction rates were 35.65 - 36. 16 mg/ mL, 60% -63.3%, 109. 5 mg/mL , 50% and 51.06 mg/mL, 66. 7%, respectively. The obtained at- tractant in this study possesses the best attractive effects against citrus fruit fly up to date.