HPLC法测定白芍药材中白芍总苷的含量

The Study on Determination of Total Glucosides of Paeony in Radix Paeoniae Alba

目的以苯甲酸为对照品，测定白芍药材中白芍总苷的含量。方法采用碱水解-HPLC法测定白芍药材中苯甲酸的含量，以公式“白芍总苷含量％=苯甲酸含量％x480．27／122．12”计算白芍总苷的含量；采用Kromasil 100-5C18 250＊46mm色谱柱，流动相为乙腈-0．1％磷酸（26：74），检测波长230nm，流速1mL·min-1，柱温30℃。结果方法重现性和稳定性均良好，苯甲酸在4．34—43．4ug·mL-1线性关系良好，回归方程为Y=59136．6879X＋4034．7289，r=1．0000（n=5），平均回收率为97．88％，RSD为2．13％（n=6）。结论该方法用于白芍药材中白芍总苷的含量测定稳定、可靠。

Objective To set up a simple and accurate method for the determination of total glucosides of paeony in Radix Paeoniae Alba（TGP） with benzoic acid as control sample. Methods Using the method of alkaline hydrolysis-HPLC, the amounts of TGP were obtained from the following formula： TGP（%）=benzoic acid（%） ×480.27/122.12. Chromatographic conditions concluded 18 alkyl silane Bonded Silica as the stationary phase, a mixture of acetonitrile - 0.1% phosphoric acid （25：75） was the mobile phase, the detection wavength was 230nm, the flow rate was 1.0 mL/min, the column temperature was 30℃. Results The calibration curves of benzoic acid were in good linearity over the range of 4.34-43.4ug/mL（r=1.000 0）. The average recovery rate was 97.88%, RSD was 2.13%（n=6）. Conclusion The method was repeatable and stable, and it can be used to determine the total glucosides ofpaeony in Radix Paeoniae Alba.