IMP3与CD44v6在甲状腺乳头状癌中的表达及临床意义

Clinical significance on expression of IMP3 and CD44v6 in papillary thyroid carcinoma

目的 探讨IMP3及CD44v6在甲状腺乳头状癌（PTC）中的表达及其临床意义.方法 应用免疫组织化学方法检测30例甲状腺乳头状癌（甲状腺滤泡型乳头状癌10例,非滤泡型乳头状癌20例）,20例甲状腺乳头状癌并颈部淋巴结转移,20例甲状腺良性组织（10例正常组织,10例结节性甲状腺肿）中IMP3和CD44v6表达水平,检测结果用Biosens Digital Imaging System vl.6专业图像分析软件进行定量分析,选择积分光密度（IOD）作为评价参数.结果 IMP3与CD44V6在PTC中的表达高于良性组织中的表达,差异有统计学意义（P ＜0.05）;IMP3与CD44v6在PTC有淋巴结转移中的表达高于无淋巴结转移组织中的表达,差异有统计学意义（P ＜0.05）;IMP3与CD44v6在PTC中的表达呈正相关（r=0.903,P＜0.05）.结论 IMP3与CD44v6在甲状腺乳头状癌中呈高表达,有作为分子标志物协助PTC的诊断及判断PTC的侵袭力及转移力的价值.

Objective To investigate expression,significance and relationship of IMP3 and CD44v6 protein in papillary thyroid carcinoma.Methods Immunohistochemical method was used to investigate the expression of IMP3 and CD44v6 protein in 30 cases PTC（10 follicular variant of papillary carcinoma cases,20 conventional papillary thyroid carcinoma cases）,20 PTC with lymph node metastasis cases,20 benign tissues of thyroid cases.Quantitative analysis of the detection was conducted by Biosens Digital Imaging System vl.6 （professional image analysis software）,and the target integral optical density （IOD） was used for the judgement parameter.Results IMP3 and CD44v6 expression in the PTC is higher than in benign group,the difference was statistically significant （P 〈 0.05） ; IMP3 and CD44v6 expression in PTC with lymph node metastasis was higher than without lymph node metastasis in the organization to express,the difference was statistically significant （P 〈 0.05） ; There was a positive correlation between IMP3 and CD44v6 expression and metastasis in PTC （r =0.903,P 〈 0.05）.Conclusions The expression of IMP3 and CD44v6 protein was closely correlated with the invasion and metastasis of PTC.IMP3 and CD44v6 protein might be considered as molecular markers for PTC.