凝胶色谱净化-高效液相色谱法测定油炸面制品中苯并(a)芘

Determination of benzo (a) pyrene in the fried noodle with Gel permeation chromatography purification and high-performance liquid chromatography

目的建立油炸面制品中苯并（a）芘的测定方法。方法样品经石油醚（沸程30℃～60℃）浸提油脂，乙酸乙酯＋环己烷（1：1）溶解，凝胶色谱（GPC）净化，溶剂转换浓缩后采用高效液相色谱法C18柱分离，以乙腈：水（95：5）作为流动相，流速1．0ml／min，检测器激发波长290nm、发射波长430nm进行检测，外标法定量。结果苯并（a）芘标准曲线在0．10—10．0μg／L范围内呈线性关系，r=0．999，回收率为90．0％-93．7％，相对标准偏差（RSD）均小于4％，方法检出限0．1μg／kg。结论该测定方法具有前处理简单、快速、准确、灵敏度高等优点，可适用于油炸面制品样品苯并（a）芘的测定。

Objective To establish the determination method of the benzo （a） pyrene in the fried flour products. Methods Samples were extracted with petroleum ether （ boiling range of 30℃～60℃ ） , were dissolved by the ethyl acetate ＋ cyclohexane （ 1 ： 1 ） and purified by gel permeation chromatography （GPC） . After con- centration, the sample was assayed by the high -performance liquid chromatography under the special condi- tions which include C18 column, mobile phase of acetonitrile - water （95： 5） , flow rate 1.0ml/min, excita- tion wavelength 290nm and emission wavelength of 430nm for detection, external standard method of quantita- tive Results BaP standard curve in 0. 10 - 10. 0μg / L range was linear, r = 0. 999, the recovery was 90. 0% - 93.7%, RSD of the method was less than 4%, the detection limit was 0. 1μg/kg. Conclusions The method is simple, fast, accurate, high sensitivity and suitable for determination of BaP in the fried flour products.