摘要
This study was carried out in the Wistar rats to investigate the effect of Tang herb on the pharmacokinetics of the antiretroviral drug lopinavir （LPV） following single oral administration. Rats were randomly divided into LPV group （36 mg/kg） and LPV combining with Tang herb group （LPV： 36 mg/kg, Tang herb： 864 mg/kg）. The blood concentrations of lopinavir at 0, 0.5, 1, 2, 4, 8, 10, 12, 14, 16, 22 h after administration were determined by HPLC and the pharmacokinetic parameters were investigated. The results showed that Tang herb significantly increased the Cmax and A UC （P〈0.05） while slightly increased the t1/2z and Tmax of lopinavir （P〉0.05）. The present study suggests that Tang herb may delay the absorption, increase the time to reach the maximum concentration, and improve the bioavailability of LPV. Further evaluation of the possible interaction mechanism between Tang herb and LPV needs to be studied.
本研究利用大鼠模型探索抗艾滋病辅助治疗中成药唐草片对抗病毒药洛匹那韦药代动力学的影响。大鼠随机分为洛匹那韦组(36 mg/kg)和唐草片联用洛匹那韦组(LPV:36 mg/kg,Tang herb:864 mg/kg),灌胃给药,高效液相色谱法测定给药前后0,0.5,1,2,4,8,10,12,14,16,22小时的血药浓度,计算药动学参数。结果显示:唐草片与洛匹那韦合并用药后,洛匹那韦的峰浓度Cmax和曲线下峰面积AUC显著提高(P<0.05)、半衰期t1/2z和达峰时间Tmax延长(P>0.05)。本研究提示唐草片可延迟洛匹那韦的吸收、推迟达峰时间、提高生物利用度,具体作用机制需要深入研究,临床用药需要考虑相互作用。
基金
Major New Drug Discovery Science and Technology Group(Grant No.2012ZX09303013)
