高脂培养抑制LPS诱导的HSC-T6细胞活化

The Fat Culture Inhabited LPS-Induced Activation of HSCs-T6 Cells

目的:鉴定高脂培养对肝星形细胞活化的影响。方法:培养HSC-T6细胞系,加入含有游离脂肪酸的高脂培养基处理,利用LPS处理活化,通过检测α-SMA的表达分析星形细胞的活化程度,通过Q-PCR分析HSCs细胞胶原的表达,通过Q-PCR实验分析LPS相关通路靶基因表达情况情况。结果:高脂培养能够抑制LPS诱导的HSC-T6细胞增殖,降低HSC-T6细胞α-SMA和胶原I和TIMP-1表达的水平,Q-PCR的分析表明,高脂培养能够抑制HSCs活化后的NF-κB通路下游靶基因MCP-1和IL-6的表达。结论:在体外培养实验中,高脂培养能够抑制LPS诱导的HSC-T6细胞活化。

Objective： To investigate the effect of high-fat medium on the activition of hepatic stellate cells. Methods： HSC-T6 cell line was cultured, and treated with high-fat medium which containing flee fatty acid, activated with LPS. The degree of activation was analyzed by detecting α-SMA expression. The expression of collagen-I and TIMP-1 was analyzed by Q-PCR, and the activation of transcription factor which related to LPS pathway was analyzed by Q-PCR assay. Results： High-fat medium was able to inhibit the activation of HSC-T6 cells which induced by LPS, reduced the expression of collagen level in the HSC-T6 cells. Q-PCR analysis showed that the high-fat medium inhibited the expression of MCP-1 and IL-6 which were the target genes of NF-KB in activated HSCs. Conclusion： In vitro culture experiments, high-fat medium can inhibit the activation of HSC-T6 cells which induced by LPS.