hBMP-7基因转染对原代培养兔髓核细胞生物学活性的影响

Effect of hBMP-7 Gene Transfection on Biological Activity of Primary Cultured Rabbit Nucleus Pulposus Cells

目的:探讨原代培养的兔髓核细胞转染腺病毒载体介导的人骨形态发生蛋白-7(hBMP-7)基因后对其生物学活性的影响。方法:根据兔髓核细胞转染方式的不同,分为hBMP7组、LacZ组、未转染组三组,hBMP7组采用腺病毒载体介导的hBMP7进行转染,LacZ组转染LacZ基因,未转染组不转染任何基因。比较三组细胞增殖、硫酸糖胺多糖(GAG)产量、Ⅱ型胶原产量有无差异。结果:处理方式和转染后时间对细胞增殖、GAG产量、Ⅱ型胶原产量有交互作用(P<0.05),hBMP7组细胞增殖、GAG产量、Ⅱ型胶原产量高于LacZ组、未转染组,差异有统计学意义(P<0.05),LacZ组和未转染组细胞增殖、GAG产量、Ⅱ型胶原产量差异无统计学意义(P>0.05)。结论:hBMP-7基因转染可提高髓核细胞增殖能力,促进其产生细胞外基质。

Objective： To investigate the primary cultured rabbit nucleus pulposus cells transfected with adenovirus vector mediated human bone morphogenetic protein-7 effect on its biological activity after （hBMP-7） gene. Methods： Depending on nucleus pulposus cells transfected way, into hBMP7 group, LacZ group, untransfected three groups, hBMP7 group using adenoviral vector-mediated transfection hBMP7, LacZ transfected with LacZ gene, not transfected genes without any transfection. Comparison of the three groups of cell proliferation, sulfate glycosaminoglycan （（GAG） production , whether the difference in type Ⅱ collagen production. Results： After treatment and transfection time on cell proliferation, GAG production, type Ⅱ collagen production had interaction （P〈0.05）, hBMP7 cell proliferation, GAG production, type Ⅱ collagen production is higher than LacZ group, the non-transfected group, the difference was statistically significant （P〈0.05）, cell proliferation LacZ group and untransfected group, GAG production, Ⅱ collagen production was no significant difference （P〉0.05）. Conclusion： hBMP-7 gene transfection can increase the proliferation of nucleus pulposus cells and promote their produce extracellular matrix.