熊果苷对小鼠脑缺血再灌注损伤的影响

The Effect of Arbutin on Mice Brain Cells after Ischemia-reperfusion Injury

目的:本研究旨在探讨中药熊果苷对缺血再灌注损伤后脑细胞的影响,为中药熊果苷的临床应用提供理论依据。方法:昆明种小鼠40只,随机分成4组,即空白组、模型组、药物预防组和药物治疗组。根据缺血时脑损伤原理制成脑缺血再灌注损伤模型,以TTC染色、HE染色观察细胞形态学变化,并检测脑组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及谷胱甘肽过氧化物酶(GSH-Px)活性的变化。结果:与模型组相比,药物预防组和药物治疗组分别TTC染色缺血区域都不如模型组坏死明显,HE染色显示细胞损伤程度减轻,SOD、GSH-Px活性提高有显著性差异,MDA含量减少(均P<0.05)。结论:药物熊果苷具有抗氧化作用,能有效地预防和保护脑细胞损伤。

Objective： To investigate the effect of Arbutin on mice cerebral cells after ischemia-reperfusion injury, and provide a theoretical basis for clinical application of arbutin. Methods： Mice were randomly divided into four groups which were sham operation group, ischemia reperfusion group, and two Arbutin groups （the chemoprophylaxis group and the medication group）. The TTC staining changes and the histological changes of brain tissue after ischemia-reperfusion injury in mice were detected. The content of malonic dialdehyde （MDA）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in brain tissue were also determined. Results： There was vitality injury after TTC staining（all P〈0.05 ）. SOD and GSH-Px, the antioxidant targets, significantly increased, while MDA decreased in the treatment group compared with that in the model group. Conclusions： Arbutin can effectively protect the mice brain cells with the effect of anti-oxidation.