NDRG2通过调控CD24影响肝癌细胞侵袭能力的研究

NDRG2 Expression Regulates CD24 and Invasion ability of Hepatocellular carcinoma Cells

目的:阐明NDRG2(N-Myc downstream-regulated gene 2)在肝癌细胞中对CD24的调控及其对乳腺癌细胞侵袭能力的影响。方法:Western blot检测低转移性的肝癌细胞Huh7、高转移性的肝癌细胞系MHCC97h及正常人肝细胞系L-02中NDRG2和CD24的表达;通过腺病毒载体上调MHCC97h细胞中NDRG2的水平,或利用siRNA下调Huh7细胞中NDRG2的表达,检测CD24的变化以及细胞侵袭能力的改变。结果:MHCC97h细胞中NDRG2基因和蛋白的表达水平低于Huh7细胞,而CD24的表达水平高于Huh7细胞;在MHCC97h细胞中上调NDRG2可以抑制CD24的表达并抑制其侵袭能力,而在Huh7细胞中下调NDRG2的表达可以提高CD24的水平及细胞的侵袭能力。结论:NDRG2可能通过影响CD24参与调控肝癌细胞的侵袭能力。

Objective： To explore the regulative role of N-Myc downstream-regulated gene 2 （NDRG2） on CD24 expression and the invasion ability of hepatocellular carcinoma cells. Methods： The mRNA and protein expressions of CD24 and NDRG2 in MHCC97H, Huh7 and L-02 cells were analyzed. Changes in cell invasion were detected when NDRG2 was up- or down-regulated by adenovirus or siRNA. The expression pattern of NDRG2 and CD24 in HCC cells and the relationship between NDRG2 and invision features were analyzed. Remits： NDRG2 expression was negatively correlated with malignancy in HCC. CD24 protein decreased when the hepatocellular cacinoma cell MHCC97H was infected by Ad-NDRG2. CD24 protein increased when the hepatocellular cacinoma cell Huh7 was transfected by siRNA-NDRG2. The up-regulation of NDRG2 decreased CD24 expression and cell invasion. By contrast, the down-regulation of NDRG2 enhanced CD24 expression and invasion. Conclusion： The results suggest that NDRG2 exerted anti-tumor activity by regulating CD24, which mediates that cell-cell interaction, tumor proliferation and adhesion.