江苏省2012年柯萨奇病毒A组16型基因特征分析

Analysis on genetic character of Coxsackievirus A16 strains isolated from Jiangsu Province in 2012

目的：了解江苏省2012年柯萨奇病毒A组16型（CA16）分离株VP1区基因特征。方法：选取江苏省2012年14株CA16病毒分离株,用1对特异性引物进行VP1区核苷酸序列扩增,对扩增产物进行测序,利用生物信息学软件对序列进行分析,结果与CA16参考株序列进行同源性比较并构建基因进化树。结果：14株CA16分离株VP1区核苷酸和氨基酸同源性分别为90.5%~100.0%和98.7%~100.0%,与CA16国际标准株G10的核苷酸和氨基酸同源性分别为75.5%~76.7%和91.6%~92.3%。江苏省2012年的CA16分离株全部属于B1基因亚型,同时包含B1a 和B1b 两条进化分支。结论：江苏省2012年有CA16病毒的B1a与B1b两种进化分支共同存在与循环,且呈现以B1b基因亚型为优势型别。B1a基因亚型为辅助型别的传播模式;无论是优势型别还是辅助型别,均各自呈现密切的亲缘关系。

Objective：To analyze the genetic characteristic of VP1 gene of coxsackievirus A16 （CA16） strains isolated from Jiangsu Province in 2012. Methods：A total of 14 CA16 strains isolats were selected to conduct VP1 gene amplification by using specific primers,and then sequenced and analyzed by bioinformatics software. The homology and phylogenetic analysis were conducted between the 14 isolates and other CA16 strains downloaded from Genbank,based on the sequence of VP1 gene. Results：The sequence analysis showed that the homogeneity of the nucleotide and amino acid of VP1 gene were 90.5%~100% and 98.7%~100% among 14 isolated CA16 strains,respectively. The homologies of nucleotide and amino acid of VP1 gene were 75.5%~76.7% and 91.6%~92.3% among the prototype（G10）,respectively. All of the CA16 isolated strains from Jiangsu Prorice in 2012 were genogroup B and they were divided into 2 branches：B1a and B1b. Conclusion：The transmission mode was showed to be the dominant subgenotype of B1b and the secondary subgenotype of B1a,and co-circulated together. Both two subgenotypes showed close phylogenetic relationship in their own subgenotype,respectively.