RP-HPLC法测定维药白花丹中白花丹醌的含量

Determination of plumbagin in Uygur medicine Plumbago zeylanica L.by RP-HPLC

目的：建立维吾尔药材白花丹中白花丹醌含量测定方法。方法采用反相高效液相色谱法，色谱柱为Waters XTerra RP C18（250 mm ×4．6 mm ，5μm）；流动相为甲醇-水溶液；体积流量为1．0 mL ·183; min-1；检测波长为270 nm ；柱温25℃。结果在上述条件下，白花丹醌进样量在0．0286～0．4576μg范围内呈良好的线性关系，加样回收率为100．4％，RSD为1.2％。结论该方法简便、准确、灵敏度高、重复性好，能有效测定白花丹药材中白花丹醌的含量。

Objective To establish a method for the determination of plumbagin in Plumbago zeylanica L ..Methods Plumbagin was determined by RP-HPLC on a Waters XTerra RP C18 （250 mm ＆#215; 4 .6 mm ,5μm） column with methanol-water as the mobile phase . The flow rate was 1 .0 mL ＆#183; min-1 ,the detection wavelength was 270 nm ,and the column temperature was 25 ℃ .Results The cal-ibration curve of plumbagin was linear within the range of 0 .028 6-0 .457 6 μg and the average recovery was 100 .4% （RSD=1.2% ） .Conclusion The method is simple ,accurate ,sensitive and reproducible .It could be used for the determination of plumba-gin in Plumbago zeylanica L ..