高效液相色谱法测定多维元素片中泛酸的含量

Determination of pantothenic acid in Vitamins with Minerals Tablets by HPLC

目的：建立复合多维元素片中泛酸的含量测定方法。方法高效液相色谱法，C18色谱柱（250 mm ＆#215;4．6 mm ，5μm ）；流动相：乙腈-1 mL ＆#183; L -1磷酸水溶液（5∶95）；流速：1．0 mL ＆#183; min-1；柱温：25℃；检测波长：200 nm。结果泛酸质量浓度在1．32～132．32μg ＆#183; mL -1范围内具有良好的线性关系，相关系数 r＝0．9999，平均回收率为99．3％，RSD为1．4％（n＝12）。结论该方法快速简便，结果准确，适用于多维元素片中泛酸含量测定。

Objective To develop a method for determining the content of pantothenic acid in Vitamins with Minerals Tablets .Meth-ods The HPLC was carried out on a C18 （25 mm ＆#215; 4 .6 mm ,5 μm） column ;the mobile phase consisted of acetonitrile-1 mL ＆#183; L -1 phosphoric acid solution（5∶95）with flow rate of 1 .0 mL ＆#183; min-1 ;the column temperature was set at 25℃ ;the detection wave-length was set at 200 nm .Results The linearity of the method was ranged from 1 .32 to 132 .32 μg ＆#183; mL -1 （r=0 .999 9） ,and the average recovery rate was 99 .3% ,with RSD 1 .4% （n=12） .Conclusions This method is rapid ,simple ,accurate ,and applicable to the determination of pantothenic acid in Vitamins with Minerals Tablets .