超滤质谱技术筛选板蓝根中抗流感病毒的活性成分

Screening bioactive compounds inhibiting influenza virus from Isatidis Radix by ultrafiltration mass spectrometry

目的:筛选板蓝根中抗流感病毒的活性成分。方法:采用超滤质谱技术,筛选板蓝根中具有抗流感病毒的活性成分,并以体外神经氨酸酶活性实验进行验证。结果:通过超滤质谱筛选并鉴定出与神经氨酸酶结合的成分主要是精氨酸、告依春和腺苷,其结合常数分别为(36.23±1.12)%,(32.54±1.02)%,(9.38±0.47)%;该结果与体外酶活性实验结果一致。精氨酸、告依春的IC50分别为(1.16±0.02),(1.20±0.02)g·L-1,腺苷的IC50大于500 g·L-1。结论:精氨酸和告依春可能是板蓝根抗流感病毒的活性成分,为板蓝根质量评价指标的选择提供了科学依据;亦为中药药效物质的发现提供了一种适宜技术。

In vitro neuraminidase inhibition assays and uhrafiltration liquid chromatography with diodearray detector coupled to time of flight mass spectrometer （UPLC-DAD-TOF-MS） were combined to screen bioactive compounds inhibiting neuraminidase from Isatidis Radix. By comparing the compounds from Isatidis Radix befo,＇e and after ultrafihration, we found that arginine, goitrin and adenosinea can bind with neuraminidase, and the binding degree of the three compounds were （36. 23 ±1.12）% , （32. 54 ± 1.02）% and （9.38 ± 0. 47 ） %, respectively. The ICs0 of arginine and goitrin were （ 1.16 ± 0. 02 ）, （ 1.20 ± 0. 02 ） g . L^ -1, respectively. While the ICs0of adenosinea was higher than 500 g . L^- 1. The results showed that arginine and goitrin might be the main compounds with antiviral activity of Isatidis Radix. This study may provide a useful method for the screening of bioactive compounds and quality control of Isatidis Radix.