人参皂苷Ro碱水解动力学研究及水解产物结构解析

Study on hydrolysis kinetics of ginsenoside-Ro in alkaline medium and structural analysis of its hydrolyzate

研究人参皂苷Ro在碱性条件下的稳定性,考察了不同温度不同pH下人参皂苷Ro的水解情况,结果发现水解符合一级动力学反应。水解速率与水解温度和水解体系的pH相关,相同的水解温度,pH越高,水解越快;相同的pH,温度越高水解越迅速。对水解产物进行分离,通过质谱、核磁等手段鉴定水解产物为姜状三七皂苷R1(zingibroside-R1)。该研究为评价人参皂苷Ro环境特性提供一定依据,也为姜状三七皂苷R1的制备提供一种方法。

The ginsenoside-Ro is sensitive to be hydrolyzed in an alkaline medium. This paper investigated the hydrolysis ki- netics of ginsenoside-Ro under different pH and temperature values. The results showed ginsenoside-Ro in alkaline solution followed pseudo-first-order reaction. Hydrolysis kinetics of ginsenoside-Ro has not been reported previously. The hydrolysis rate was independent of initial concentration. On the basis of UFLC-MS/MS, NMR, as well as chemical evidence, the structure of hydrolyzate was assigned as 3-O- [ β-D-glucuronopyranosyl- （ 1 →2 ） -fl-D-glucopyranosyl ] -oleanolie acid.