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必需氨基酸上调乳腺腺泡中L型氨基酸转运载体1的表达 被引量：1

Essential Amino Acid Upregulates L-type Amino Acid Transporter 1 Expression in Mammary Acinus
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摘要 为探讨泌乳乳腺中必需氨基酸与L型氨基酸转运载体1(L-type amino acid transporter 1,LAT1)表达之间的关系,本研究通过体外构建奶牛乳腺泌乳模型,采用免疫印迹方法检测生理浓度的必需氨基酸对乳腺腺泡中LAT1及其辅因子4F2重链(4F2heavy chain,4F2hc)表达变化的影响。结果表明,生理浓度的必需氨基酸显著上调体外培养的奶牛乳腺腺泡中LAT1和4F2hc的表达,提示LAT1是奶牛乳腺上皮细胞内负责转运必需氨基酸的主要转运载体,LAT1和4F2hc的表达受必需氨基酸的诱导。 To investigate the effect of essential amino acids on LAT1 expression in lactation mammary gland, the lactation mammary acini were cultured and LAT1 and 4F2hc expression were detected by Western blotting. The results showed that es- sential amino acids upregulated LAT1 and 4FZhc expression significantly in lactation mammary acini of dairy cow. It revealed that LAT1 was the mainly amino acid transporter in lactation mammary gland. The expression of LAT1 and 4F2hc was induced by essential amino acids.
作者 林叶 侯晓明 田雷 齐帮若 李庆章
机构地区 东北农业大学乳品科学教育部重点实验室
出处 《中国畜牧兽医》 CAS 北大核心 2014年第3期83-86,共4页 China Animal Husbandry & Veterinary Medicine
基金 2010年高等学校博士学科点专项科研基金联合资助课题(新教师类联合)(20102325120018) 2012年黑龙江省政府博士后科研启动金(LBH-Q12157) 东北农业大学科技人才启动基金项目(校博士启动基金)
关键词 乳腺腺泡 必需氨基酸 L型氨基酸转运载体1(LAT1) 4F2重链(4F2hc) mammary acinus essential amino acid LAT1 4F2hc
分类号 Q517 [生物学—生物化学]
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参考文献12

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二级参考文献22

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中国畜牧兽医
2014年 第3期

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