牛粪中纤维素分解菌的分离鉴定及其产酶条件优化研究

Study on Isolation,Identification and Enzyme-producing Conditions Optimized of Cellulolytic Bacteria in Ox Dung

本试验通过对牛粪进行富集培养、分离纯化,利用刚果红染色法和摇瓶复筛得到1株产酶活较高的纤维素分解菌N12,经形态观察、生化鉴定和16SrRNA遗传学鉴定为芽孢杆菌属的短小芽孢杆菌(Bacillus pumilus),并对其产酶条件进行了初步优化。结果表明,菌株N12以0.5%玉米粉为碳源、1%酵母粉为氮源、初始pH 3.0、42℃摇瓶培养54h产酶活最高。

A strain of highly cellulolytic decomposing bacteria was obtained by the congo red flat plate straining and shake flasking method screened from the ox dung which named N12. It had been identified as Bacillus which belonged to Bacillus pumilus sp. by morphologic characteristics observation,physiology and biochemical experiment and also 16S rRNA genetic ana- lyze. The conditions of producing cellulase on strain N12 were also been studied in this test. When the contents of carbon and ni trogen sources were 0. 5% maizena and 1 % yeast powder, and also the nitial pH was 3.0, the fermentation temperature was 42℃ ,at the same time the fermentation time was 54 h,it had the highest enzyme activity.