摘要
为了研究藏獒体细胞生物学特性和保存藏獒遗传资源,采用组织块法建立了2株藏獒成纤维细胞系,对体外培养的细胞形态、增殖能力和部分生物学特性进行了分析。结果表明,体外培养的藏獒细胞呈现典型的成纤维细胞形态,生长良好,增殖能力强,测定的细胞群体倍增时间为46.1h;经免疫荧光染色,细胞表达波形蛋白;F5代细胞正常二倍体核型率为91.4%,藏獒核型2n=78,X染色体为亚中着丝粒染色体,Y染色体为端着丝粒染色体。本研究所建立的藏獒体细胞系为开展藏獒体细胞核移植研究提供了材料。
n order to study biological characteristics of Tibetan Mastiff somatic ceils and preserve genetic resource, two fi- broblast cell strains from Tibetan Mastiff were established by tissue culture. In vitro culture cell morphology, hyperplasia abil- ity and some biological characteristics were researched. Tibetan Mastiff ceils in vitro culture appeared typical morphology of fi- broblast cells, grew well and had strong hyperplasia ability. Population doubling time of the cells was 46.1 h. Vimentin ex- pression in the cells was confirmed by immunofluorescence staining. The rate of F5 cells with normal diploidy （2n= 78） was 91.4%. Moreover, longer X chromosome belonged to submetacentric chromosome, but Y chromosome did telocentric chromo- some. Tibetan Mastiff cell strains eslablished in the study offered the materials for the Tibetan Mastiff somatic cell nuclear transfer research.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第3期156-161,共6页
China Animal Husbandry & Veterinary Medicine
基金
中国科学院2012年“西部之光”人才培养计划项目“藏獒繁殖生物技术研究”(科发人教字[2012]179号)
财政部战略生物资源科技支撑专项“青藏高原羊模式动物及基地建设”(KSCXZ-YW-Z-0951)
关键词
藏獒
细胞培养
成纤维细胞
免疫荧光染色
Tibetan Mastiff
cell culture
fibroblast cell
immunofluorescence staining
