摘要
通过对菊花茎段采用不同的消毒处理,然后进行诱导、增殖和生根试验,建立了菊花离体快繁体系:消毒灭菌处理以70%酒精浸泡10s后,在0.1%升汞溶液中浸泡3min为最佳;带腋芽茎段诱导的最适宜培养基是MS+6-BA2.0 mg·L-1+ NAA0.1mg·L-1;腋芽增殖的最适宜培养基为MS+6-BA2.0 ~3.0 mg·L-1+NAA0.1mg·L-1;利用不带腋芽的茎段诱导愈伤组织的最适宜培养基是MS+6-BA0.5mg·L-1+ NAA0.5mg·L-1;愈伤组织再分化成丛生芽的最适宜培养基是MS+6-BA2.0 mg·L-1+ NAA0.1mg·L-1;诱导生根的适宜培养基为1/2MS+ NAA0.5 mg·L-1;生根率为100%;试管苗移栽到疏松透气的蛭石+珍珠岩+园土(1:1:1)混合基质中,成活率可达98%.
The techniques of vitro propagation for Chrysanthemum were established by adopting different sterilizing treat- ment, induction, proliferation and rooting culture mediums. The results showed that the optimum way of sterilizing treat- ment was that explants were dipped in 70% alcohol for 10s,then in 0.1% HgCl2 for 3 rain. The best culture medium for proliferation was MS + 6 -BA2.0. 3.0 mg.L-1 + NAA0.1 mg. L-l. The best euhure medium for callus induction was MS + 6 - BA0.5 mg.L-1 + NAA0.5mg . L-1. The best culture medium for callus re - differentiation was MS + 6 - BA2.0 mg. L-1 + NAA0. mg. L-1 ;The best culture medium for axillary buds induction was MS + 6 - BA2.0 mg. L-1 + NAA0. ling . L-1. Buds were placed onto the rooting medium 1/2MS + NAA0.5 mg. L-1 , induction rate of roo- fing was 100%. There was no strict demand for matrix, when the plantlets were transplanted to medium (vermiculite : pearlite: soil = 1:1:1), the survival rate could reach 98%.
出处
《信阳农业高等专科学校学报》
2013年第4期91-94,共4页
Journal of Xinyang Agricultural College
关键词
菊花
组织培养
快速繁殖
Chrysanthemum
tissue culture
rapid propagation