全反式维甲酸诱导膀胱癌T24细胞株凋亡的研究

Induction of apoptosis by all trans-retinoic acid in bladder tumor T24 cell line

目的 :探讨全反式维甲酸 (ATRA)对人膀胱癌 T2 4细胞株的凋亡诱导作用。方法 :应用荧光显微镜、流式细胞仪及 DNA琼脂糖凝胶电泳等技术研究 T2 4细胞凋亡。结果 :用 3× 10 - 6 m ol/ L 的 ATRA处理 T2 4细胞6天 ,荧光显微镜下计数 T2 4细胞凋亡率为 15 .2 0 % ,对照组为 0 .0 1% (P <0 .0 5 ) ;流式细胞仪测定 T2 4细胞凋亡率为 15 .31% ,对照组为 1.49% ;T2 4细胞 DNA琼脂糖凝胶电泳呈现凋亡特征性梯形条带。结论 :ATRA对人膀胱癌 T2 4细胞株有凋亡诱导作用 ,为

Purpose:To explore the all trans retinoic acid(ATRA) on induction of apoptosis in human bladder transitional cell carcinoma cell line T24.Methods: The apoptosis of T24 cell line was studied by fluorescence microscopy, flow cytometry and DNA agarose gel electrophoresis.Results:After T24 cells were treated by 3×10 -6 mol/L ATRA six days, the apoptotic rates of T24 cells were 15.20 % in treated group and 0.01 % in control group respectively under fluorescence microscopy(P< 0.05 ); the apoptotic rates of T24 cells were 15.31 % in treated group and 1.49 % in control group separately on flow cytometry ; a characteristic DNA “ladder” on agarose gels was observed in treated group.Conclusions:The apoptosis of human bladder transitional cell carcinoma cell line T24 can be induced by ATRA.