低氧训练后AMPKα2转基因鼠骨骼肌谷胱甘肽氧化还原状态的变化

Changes of Glutathione Redox in Skeletal Muscles of AMPKα2 Over-expression Mice after Hypoxia Training

目的:观察低氧训练后AMPKα2转基因鼠骨骼肌谷胱甘肽氧化还原状态的变化。方法:C57BL/6J野生小鼠、AMPKα2高表达转基因鼠各20只,随机分为常氧安静组和低氧训练组,每组各10只小鼠,进行4周的低氧运动训练,第4周后取小腿骨骼肌,采用生化比色的方法测定2组小鼠GSH水平,GSH/GSSG比值以及GR和GCL活性的变化。结果:(1)AMPKα2转基因鼠四周的低氧耐力训练后GSH/GSSG显著性增加(P<0.05),并且与野生鼠相比,低氧耐力训练后GSH/GSSG比值具有显著差异;(2)低氧训练后野生鼠和AMPKα2转基因鼠骨骼肌GR活性均显著增加,但两鼠间没有显著差异;AMPKα2转基因鼠低氧训练前后GCL活性均显著高于野生鼠。结论:四周低氧训练后,AMPKα2转基因鼠骨骼肌GSH/GSSG比值显著增加,说明低氧训练促进了AMPKα2转基因鼠谷胱甘肽氧化还原平衡状态。该比值的增加,与AMPKα2转基因鼠本身骨骼肌GSL活性增加和低氧训练增强GR活性有关。

Objective： This paper aims to investigate changes of glutathione redox in skeletal muscles of AMPKα2 over-expression mice after hypoxia training. Methods ： Two-month old C57BL/6J wild-type （WT） mice （ n = 20 ）, α2- AMPK over-expression（OE） mice（ n =20） were randomly divided into two groups： normoxia control group and hypoxia training group. After four weeks hypoxia endurance training, calf muscles were collected to measure the GSH level, GSH / GSSG ratio and the activity of GR and GCL by chromatography method. Results ： （ 1 ） After four weeks of hypoxia endurance training, the ration of GSH / GSSG increased significantly in OE mice （ p 〈 0. 05 ） , and this ratio had significant difference compared with WT mice. （ 2 ） GR enzyme activity increased in both groups after hypoxia training, but there was no difference between two groups. Besides, the GCL enzyme ac- tivity in OE mice was higher than in WT mice before and after training,. Conclusions ： The result, hypoxic training increased GSH / GSSG ratio in AMPKα2 mice, suggested that hypoxic training improved the balance of glutathi- one in AMPKα2 mice. The increase of GSH / GSSG ratio was related to increased GSL activity in skeletal muscles and decreased GR activity in AMPKα2 mice.