补脾益气中药对ICAM-1的表达和PLA_2含量的影响

Effect of Prescription and Medicine of Strengthening Spleen and Benefiting Vital Qi on Expression of ICAM-1 and Contents of PLA_2

目的 :探讨细胞间粘附分子 (ICAM- 1)和磷脂酶 A2 (PL A2 )在哮喘发病过程中的作用及机理。方法 :采用卵蛋白等致敏大鼠哮喘模型 ,观察支气管肺泡上皮细胞 ICAM- 1的表达情况及支气管肺泡灌洗液 (BAL F)中PL A2 和血浆中 PL A2 活性变化 ,以及 BAL F中细胞总数及细胞分类情况 ,及三者之间的关系。结果 :与对照组比哮喘组支气管上皮细胞 ICAM- 1表达显著增强 ,BAL F中 PL A2 活性显著性增高 ,血浆中 PL A2 活性也增高 ,但未达显著水平 ;哮喘组 BAL F中细胞计数显著升高 ,尤其是嗜酸性粒细胞增多更为显著。将哮喘组中 ICAM- 1的阳性面积、BAL F中 PL A2 活性和 BAL F中细胞计数进行相关分析 ,结果三者显著相关。结论 :ICAM- 1和 PL A2 在哮喘大鼠的气道炎症细胞浸润过程中有很重要的作用 ,二者之间又有很密切的联系。

Objective:Inquire into the action and mechanism of ICAM-1 and PLA 2 in the course of asthmatic incidence,and the effect to them by the prescription and medicine of strengthening spleen and benefiting Vital Qi.Methods:Choose the model white rat of asthma sensitized with OA,observe the expression of ICAM-1 of the epithelial cell on the bronchial pulmonary alveolus,the active change of PLA 2 in BALF and blood plasma,and the quantity and category of the cells.Results: Compare with the contrast group,in the asthmatic group,the expression of bronchial epithelial cells ICAM-1 becomes strong obviously.The activity in BALF increases much evidently, the activity of PLA 2 in blood plasma also increases but it does not reach marked level.The cell count rises obviously and the increase of the eosinophilic granulocyte is especially more evident.We analyze the positive area of ICAM-1,the activity of PLA 2 and the cell count in BALF.They are related closely.The prescription and medicine of strengthening spleen and benefiting Vital Qi can notably reduce the expression of ICAM-1 and the contents of PLA 2 in BALF.Conclusions:ICAM-1 and PLA 2 play an very importent role in the course of inflammatory cell infiltration in asthmatic white rat's airway.Meanwhile they are closely related with each other.The prescription and medicine of strengthening spleen and benefiting Vital Qi can reform the airway state by reducing the contents of ICAM-1 and PLA 2.