序贯培养在体外受精-胚胎移植技术中的应用

Application of sequential culture in assisted reproductive technique

目的 试用序贯培养方法延长胚胎体外培养时间 ,以获得 8细胞胚及囊胚进行移植 ,提高体外受精 胚胎移植 (IVF ET)的成功率。方法 将行IVF ET的 16 1例患者共 182 3个卵子分为序贯培养 (使用P1液、B液系列培养液 ,47例、47个周期 )与传统培养 (114例、114个周期 )两组进行培养 ,分析受精率、卵裂率、卵裂速度、优质胚胎比率及临床妊娠率 ,采用 χ2 检验进行统计学分析。结果 序贯培养组卵裂率、优质胚胎比率、胚胎着床率及妊娠率分别为 97 9%、6 4 4%、19 1%和 46 7% ,传统培养组分别为 89 7%、40 2 %、11 0 %和 2 8 2 % ,两组比较 ,差异有显著性 (P <0 0 5 )。结论 采用适合的培养液进行序贯培养 ,更适合于卵子及胚胎的体外发育 。

Objective To investigate the effects of sequential culture technique on the outcomes of in vitro fertilization embryo transfer( IVF ET) Methods Sequential culture media were used in 47 IVF ET cycles, and conventional medium in 114 cycles The fertilization rate, cleavage rate and speed, good embryo rate and pregnancy rate were compared between these 2 groups Results There were significantly higher embryo cleavage and quality score rate, pregnancy rate (97 9%, 64 4%, 46 7%) in sequential media group as compared those in conventional medium group (89 7%, 40 2%,28 2%) Conclusion Sequential culture system is more suitable for the development of cultured human oocytes and embryos