瘦素对人卵巢黄素化颗粒细胞雌、孕激素分泌的体外实验研究

Effects of leptin on estradiol and progesterone production by human luteinized granulosa cells in vitro

目的 探讨瘦素在体外对人卵巢颗粒细胞雌激素和孕激素生成的影响。方法 将来自体外受精 胚胎移植 (IVF ET)的卵巢黄素化颗粒细胞纯化后 ,在不同浓度瘦素 (0、10、30、10 0、30 0ng/ml)和人绝经期促性腺激素 (hMG ,0、0 1、0 2、0 5、1、2、5、10IU/ml)单独或联合作用下进行体外培养 ,收集培养液 ,采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。结果 不同浓度的瘦素对雌二醇、孕酮的生成无影响 ;hMG为 5IU/ml时 ,雌二醇水平平均为 2 36× 10 -11mol/L ,加入不同浓度瘦素 (10、30、10 0、30 0ng/ml) ,雌二醇的生成均受到明显的抑制 ,随瘦素浓度增加 ,雌二醇水平逐渐降低(P <0 0 5 ) ,孕酮水平无明显变化 ;hMG浓度小于 0 5IU/ml时 ,瘦素对雌二醇生成无影响 ;hMG浓度大于 0 5IU/ml、瘦素为 10 0ng/ml时 ,雌二醇水平低于不加瘦素的对照 (P <0 0 5 )。结论 瘦素参与卵巢颗粒细胞激素生成的调节 。

Objective To investigate the effects of leptin on steroidogenesis of human luteinized granulosa cell in vitro Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0 1, 0 2, 0 5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml For 2 days the media were collected for estradiol and progesterone measurements Results Addition of leptin alone did not alter estradiol and progesterone production ( P> 0 05) by human luteinized granulosa cells Leptin of 10～30 ng/ml concentrations caused a dose dependent inhibition of estradiol production ( P< 0 05) while greater than 0 5 IU/ml of hMG were added There was no effect of leptin on hMG stimulated progesterone production ( P> 0 05) Conclusion Leptin can directly inhibit hMG stimulated estradiol production by human luteinized granulosa cells in vitro, but has no effect on progesterone production Leptin may play an important role in follicle development and luteinization