超排卵时卵泡发育数与颗粒细胞胰岛素样生长因子Ⅰ受体的表达

Relationship between follicular development and insulin like growth factor Ⅰ receptor in stimulated cycles

目的 探讨超排卵周期卵泡发育数与颗粒细胞胰岛素样生长因子Ⅰ受体 (IGF ⅠR)表达的关系。方法 根据取卵时卵泡发育数不同 ,将卵巢对超排卵药物的反应分为低反应型 (卵泡数≤3个 )、中反应型 (卵泡数 4～ 13个 )和高反应型 (卵泡数≥ 14个 ) ,分别为 11、16、12例。采用逆转录聚合酶链反应 (RT PCR)和蛋白印迹 (Western blot)法分别测定 3种反应类型的卵泡颗粒细胞IGF ⅠRmRNA的表达量和IGF ⅠR的含量。结果 低反应型IGF ⅠRmRNA表达量显著低于中、高反应型[相对积分值分别为 1 70± 0 2 3( x±s,下同 )、2 92± 0 49和 4 2 2± 1 5 0 ,P <0 0 5 )。低反应型与中反应型及高反应型相比 ,IGF ⅠR含量也减少 (相对吸光度分别为 1 32± 0 31、2 0 1± 0 72和 4 39±2 30 ) ,差异有显著性 (P <0 0 5 )。结论 颗粒细胞IGF

Objective To investigate the number of follicles and insulin like growth factor I receptor (IGF ⅠR) in stimulated cycles Method IGF ⅠR mRNA and IGF ⅠR in granulosa cells obtained during oocyte retrieval were respectively measured by reverse transcript polymerase chain reaction (RT PCR) and Western blot technique Results The expression of IGF ⅠR mRNA in poor responders (follicles≤3) was much lower than in normal responders (follicles 4～13) and high responders (follicles≥14) (1 70±0 23, 2 92±0 49,4 22±1 50 respectively) Similar results were obtained for IGF ⅠR (1 32±0 31, 2 01±0 72, 4 39±2 31 respectively) Conclusion The expression of IGF ⅠR correlated with the effects of gonadotropin hormone on follicular development