富硒枸杞子对临床常见分离耐药菌体外抑菌活性的研究

Antibacterial activity in vitro of elenium enriched wolfberry fruit produced in Ningxia on six kinds of drug resistance bacteria

目的探讨宁夏富硒枸杞子对6种临床常见分离耐药菌(金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌、铜绿假单胞菌、克柔假丝酵母菌、白色假丝酵母菌)体外抑菌活性的影响。方法采用微量稀释法及滤纸片扩散法,测定不同浓度的宁夏枸杞子对几种常见细菌和真菌的抑制效果,设置1.00、0.75、0.50、0.25g/mL 4种枸杞子浓度和空白对照组共5组,测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果不同浓度宁夏枸杞子提取物对耐药菌均有一定的抑菌效果,空白对照组,0.25、0.50、0.75、1.00g/mL枸杞子浸出液对大肠埃希菌的抑菌环分别为0.00、(22.78±0.31)、(23.80±0.29)、(27.57±0.47)、(30.89±0.51)mm;对铜绿假单胞菌的抑菌环分别为0.00、(23.07±0.45)、(24.16±0.49)、(29.34±0.39)、(35.16±0.34)mm,并对铜绿假单胞菌绘制了生长曲线。不同浓度枸杞子提取物对大肠埃希菌和铜绿假单胞菌的抑菌作用强于其他4种细菌。结论枸杞子不同浓度提取物对临床分离耐药菌有明显的体外抑菌效果,为进一步全面开发宁夏富硒枸杞子的药物价值提供了依据。

Objective Discuss the antibacterial activity in vitro of Ningxia selenium enriched wolfberry on 6 kinds of drug resistance bacteria which were common in cilinic（Staphylococcus aureus ,Staphylococcus aureus ,Escherichia coli ,Pseudomonas aeruginosa ,Candida krusei ,Candida albicans） .Methods Microdilution method and filter paper dispersion method were used to determinate lixivium of clinically isolated strains of the minimal inhibitory concentration（ MIC） and minimal bactericidal concentration（MBC） ,a total of five different concentration groups include the 1 .00 ,0 .75 ,0 .50 ,0 .25 g/mL 4 kinds of medlar concentration and the blank control group were set up Results Different concentrations of medlar extracts have bacteriostatic effect on drug resistant bacteria ,bacteriostatic ring of e .coli in 0 g/mL ,0 .25 g/mL ,0 .5 g/mL ,0 .75 g/mL and 1 g/mL concentration medlar leaching liquid were respectively 0 .00 mm ,（22 .78 ±177; 0 .31）mm ,（23 .80 ±177; 0 .29） mm ,（27 .57 ±177; 0 .47） mm and（30 .89 ±177; 0 .51） mm ;Bacteriostatic ring of pseudomonas aeruginosa were respectively 0 .00 mm ,（23 .07 ±177; 0 .45） mm ,（24 .16 ±177; 0 .49）mm ,（29 .34 ±177; 0 .39） mm and（35 .16 ±177; 0 .34）mm ,and mapped the growth curve of pseudomonas aeruginosa .Bacteriostasis of different concentration medlar leaching liquid for escherichia coli and pseudomonas aeruginosa bacteriostasis was stronger than that for the other 4 kinds of bacteria .Conclusion Different concentration extracts of Wolfberry produced Ningxia have obvious bacteriostatic effect in vitro on clinical isolated drug-resistant bacteria ,which provides basis for overall exploitation of medical value in ningxia selenium-rich wolfberry .