2010～2012年聊城市手足口病患儿肠道病毒的检测结果分析

Analysis on Enterovirus in Children with Hand,Foot and Mouth Disease,Liaocheng City,2010-2012

目的了解流行高峰时聊城市手足口病的病原学特征，为手足口病的防治提供科学依据。方法收集2010～2012年临床诊断或疑似为手足口病病例的咽拭子、大便和疱疹液标本，应用实时RT-PCR法进行肠道病毒71型（EV71）、柯萨奇病毒16型（Cox16）和其他肠道病毒核酸检测。结果2010～2012年合计检测406份标本，肠道病毒阳性率为67．98％，其中EV71和Coxl6阳性率分别为29．31％和21．92％，EV71和Cox16均为阳性的1例，其他肠道病毒阳性率为16．75％；其中男性阳性率为46．31％（188／406）；女性为21．68％（88／406），男性阳性率高于女性，性别比为2．14：1，差异有统计学意义（P〈0．05）；发病年龄为5个月至7岁，0～5岁为高风险年龄组；4～8月为手足口病高发期。结论聊城地区手足口病患儿以EV71和Cox16感染为主，2010年以EV71为主要病原，2011年以Cox16为主要病原，2012年又以EV71为主出现，高发病毒株交替出现。

Objective To understand the characteristic of HFMD pathogen during epidemic peak in Liaocheng city,so as to provide the scientific basis for prevention and control of HFMD. Methods The pharyngeal swabs, stool and herpes fluid of clinical diagnosis or suspected HFMD patients from January to August during 2010-2012. were collected To dis- tinct the type of enterovirus 71,coxsachie virus 16 or other enterovirus by RT-PCR. Results The total positive rate of intestinal virus in children was 67.98 % during 2010-2012. The positive rate of EV71 specimen was 29.31~, the positive rate of Cox16 specimen was 21. 920/00 ,one case was all positive for EVT1 and Coxl6 ,and positive rate of other intestinal vi- rus was 16.75M. The positive rate for men was 46.31% （188/406） and the positive rate for women was 21.68% （88/ 406） ,the former was higher than the latter. The sex ratio for male and female was 2.14 ： 1 ,and the difference had statisti- cal significance （ P 〈0.05）. The HFMD aged 5 months to 7 years old. The high risk aged group was 0 to 5 years old. The high incidence period was from April to August. Conclusion The children with HFMD are mainly infected with EV71 or Cox16. The main pathogen respectively is EV71 in 2010, Coxl 6 in 2011 and EVT1 in 2012. The high incidence of differ- ent virus appears alternately.