胃癌及癌前病变中p53蛋白的检测及意义

Expression of p53 Protein in Gastric Precancerous Lesions and Gastric Carcinoma

目的：探讨ｐ５３蛋白表达与胃癌及癌前病变的相互关系。方法：用免疫组化染色法检测３３例肠化、２６例异型增生和２６例胃癌组织中ｐ５３蛋白的表达。结果：ｐ５３蛋白在胃癌组织中表达率最高（６１．５％），在异型增生和肠化组织中的表达率分别为３４．６％和１２．１％，组间有显著差异。各期胃癌组织中ｐ５３蛋白的表达无显著差异。结论：ｐ５３蛋白在胃癌前病变中已有阳性表达，在肠化、异型增生及胃癌组织中，其表达率依次增高；ｐ５３蛋白积累主要发生在癌前病变晚期及胃癌早期，其表达与胃癌发生密切相关。

Background/Aims: To assess the clinical significance of the expression of p53 protein in gastric precancerous lesions and gastric carcinoma, and the relation of p53 protein expression to various stages of gastric carcinoma. Methods: Accumulation of p53 protein was detected by immunohisto- chemical method. Biopsies from patients with intestinal metaplasia (n=33) and dysplasia (n=26) were obtained endoscopically, gastric carcinoma specimens (n=26) were obtained by operation. Results: The highest expression rate of p53 protein was in the gastric carcinoma tissues (61.5%). The positivity rates of p53 protein in dysplasia and intestinal metaplasia were 34.6% and 12.1%, respectively, the statistical difference was significant. There was no significant difference in p53 staining among different clinical stages of gastric carcinoma. Conclusions: The positive expression of p53 protein was found in gastric precancerous lesions. Accumulation of p53 protein occurred mainly at the advanced precancerous le- sions and early gastric carcinoma, its expression was in the increasing order of intestinal metaplasia, dysplasia and early gastric carcinoma.