中国北方汉族人群核呼吸因子-1基因+141G/T单核苷酸多态与冠心病发病的关联研究

Association of NRF-1 +141G/T Polymorphism with Coronary Artery Disease in Han Population of Northern China

目的:本研究旨在探讨IRF-1基因+141 G/T单核苷酸多态位点与中国北方汉族人群冠心病发病的相关关系。方法:本研究采用聚合酶链反应-限制性片段长度多态性对经过冠脉造影证实的冠状动脉有一条主要分支狭窄大于70%的675例冠心病患者和经过冠状动脉造影证实冠状动脉狭窄小于20%或完全正常的636例对照患者进行检验检,分析核呼吸因子IRF-1基因+141G/T单核苷酸多态位点的基因型和等位基因频率在两组间的分布情况。结果:核呼吸因子IRF-1基因+141 G/T单核苷酸多态位点三种基因型(GG型,GT型和TT型)在中国北方汉族人群冠心病组的分布频率分别为53.8%,36.2%和10.1%,在对照组的分布频率分别为45.6%,46.2%和8.2%,核呼吸因子IRF-1基因+141 G/T单核苷酸多态位点的基因型和等位基因频率分布在对照组和冠心病组之间存在统计学差异(P<0.05)。Logistic回归分别校正冠心病的其他危险因素性别、年龄、体重指数、吸烟、高血压、高脂血症、糖尿病等后,核呼吸因子IRF-1基因+141 G/T单核苷酸多态位点与中国北方汉族人群的冠心病的发病存在相关关系(P<0.05)。结论:核呼吸因子IRF-1基因+141 G/T单核苷酸多态与中国北方汉族人群冠心病的发病存在相关关系,IRF-1基因+141 G/T多态可能是中国北方汉族人群冠心病发病的独立危险因子。

Objective： The aim of the present study was to investigate the association between IRF-1 ＋141 G/T polymorphism and coronary artery disease in Han population of NOrthern China. Methods： A case-control study was conducted in 675 patients with coronary artery disease and 636 controls who had normal coronary angiograms. Polymorphic genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism. Results： The genotype frequencies in IRF-1 ＋141 G/T polymorphism conformed well to the Hardy-Weinberg equilibrium in both controls and case, and were 53.8 % （GG）, 36.2 % （GT）, 10.1% （TT） in case groul5 and 45.6 % （GG）,46.2 % （GT）, 8.2 % （TT） in the controls. There were significant differences in the genotype and allele distribution of＋141 G/T polymorphism of the IRF-1 gene between cases and controls （P^0,05）. Logistic regression analysis with adjustments for other risk factors revealed that the IRF-1 ＋ 141 G/T allele carriers significantly increases the risk of coronary artery disease compared with the non-carriers （P〈0.05）. Conclusions： This study shows that the IRF-1 ＋141 G/T polymorphism may be considered a genetic risk factor for coronary artery disease in Han population of Northern China.