丁基苯酞对大鼠心肌损伤及脂质过氧化的作用

The Effect of L-3-n-butylphthalide on Myocardial Injury Rat and Lipid Peroxidation

目的:大量的临床以及动物实验表明丁基苯酞对缺血性脑损害具有明确的保护作用,但其对于相似发病机理的心肌损伤国内外尚无研究。本课题探讨丁基苯酞对异丙肾上腺素诱导大鼠心肌损伤保护作用及机制。方法:SD大鼠48只,随机分为对照组(n=12),异丙肾上腺素组(n=12),异丙肾上腺素+Tween-80(丁苯酞溶媒)组(n=12),异丙肾上腺素+丁苯酞组(n=12)。7天后处死大鼠,测定左心室血流动力学、血清SOD活性、组织MDA含量及组织病理学观察。结果:ISO组成功的制备了心肌损伤模型,与Control组相比,ISO组左室舒张末期压(LVEDP)明显升高(P<0.01),+dp/dtmax显著下降(P<0.01),SOD活性下降,MDA含量增加,心肌坏死病理积分明显增加。丁基苯酞(NBP)能显著减轻异丙肾上腺素导致的心肌损害。与ISO组相比,ISO+NBP组LVEDP明显下降,SOD活性提高,MDA含量减少,心肌坏死病理积分明显减少。结论:丁基苯酞对异丙肾上腺素引起的大鼠缺血损伤心肌具有保护作用,其机制可能与其抗脂质过氧化有关。本研究发现一种新的具有心脏保护作用的药物,可能为心肌缺血损伤疾病的预防和治疗提供新的选择。

Objective： A number of clinical and animal experimengts show that 1-3-n-butylphthalide （NBP） has protective effect on ischemic brain damage, but it has not been studied to the similar pathogenesis of myocardial damage at home and abroad. Our study investigate the effects and mechanism of NBP on myocardial from ischemia injury treated rat. Methods： 48 health SD rats were randomly divided into four groups： Control group （Control, n=12）, isoproterenol intraperitoneal injection group （ISO, n=12）, Iso＋Tween group （NBP solvent）（Tween, n=12） and Iso＋NBP group （NBP, n=12）. After 7 days, all rats heart functions were measured and then executed. Serums were collected to detect SOD activity levels, myocardial tissue was stained by HE and tissue MDA were examined. Results： The group ISO had successfully prepared the myocardial injury model. Compared with the group Control, the group ISO left Ventricular end-diastolic pressure （LVEDP） was significantly increased （P〈0.01）, ＋dp/dtmax decreased significantly （P〈0.01）, necrosis score was significantly increased. Compared with the group ISO, the group NBP showed LVEDP level decreased obviously, the serum SOD activity improve, the content of MDA organization reduced, necrosis score significantly reduced. Conclusion： NBP shows protective effect in the experimental myocardial Ischemia injury treated. The mechanism may be related to its anti-lipid peroxidation. The study found a new cardioprotective drug, this provides a new choice for disease prevention and treatment of myocardial ischemia injury.