血清一氧化氮及一氧化氮合酶体系与轻度胃肠炎合并婴幼儿良性惊厥的相关性研究

Relation between benign infantile convulsion associated with mild gastroenteritis and serum nitric oxide and nitric oxide synthase system

目的探讨一氧化氮(NO)及一氧化氮合酶(NOS)体系在轻度胃肠炎合并婴幼儿良性惊厥(BICE)发病机制中的作用。方法收集2010年1月至2012年7月于山西省儿童医院诊治的BICE患儿40例、热性惊厥患儿26例、轮状病毒肠炎患儿14例及同期门诊体检的健康儿童30名,分别采用硝酸还原酶法、酶测定法检测血清中NO水平,总一氧化氮合酶(TNOS)水平及诱导型一氧化氮合酶(iNOS)水平。结果 BICE组患儿血清NO及iNOS水平明显高于健康儿童、热性惊厥组及轮状病毒肠炎组患儿(P<0.05),但TNOS水平各组间差异无统计学意义(P>0.05)。BICE严重组及普通组患儿血清NO水平差异无统计学意义(t=1.25,P>0.05),BICE轮状病毒阳性组与阴性组患儿血清中NO水平差异无统计学意义(t=1.12,P>0.05)。结论 iNOS增加而导致的NO水平升高,可能与BICE患者的发病有关。

Objective To investigate the roles of nitric oxide （NO） and nitric oxide synthase （NOS） system in the pathogenesis of benign infantile convulsions associated with mild gastroenteritis （BICE）. Methods Serum sam- ples were taken from 40 patients with BICE, 30 healthy children, 26 children with febrile convulsion, and 14 with ro- tavirus gastroenteritis who were admitted to Shanxi Children＇s Hospital between January 2010 and July 2012. The NO concentration, total nitric oxide synthase （TNOS） and inducible nitric oxide synthase （iNOS） were measured by nitrate reductase and enzyme determination assays method, respectively. Results The levels of serum NO and iNOS, but not TNOS （all P〉0.05）, in children with BICE were significantly higher than those of healthy children, children with febrile convulsion and those with rotavirus gastroenteritis （all P〈0.05）. No significant differences of serum NO levels could be found between subgroups with serious and ordinary BICE （t=1.25, P〉0.05）, or between children with BICE with and without rotavirus （t=1.12, P〉0.05）. Conclusion Increased NO levels resulted from higher iNOS might be one of the mechanisms responsible for the pathogenesis of BICE.