摘要
目的 :建立HPLC法测定利福平胶囊含量。方法 :采用C18柱为固定相 ,乙腈 0 .0 4mol·L-1磷酸二氢铵 ( 4 0 :70 )为流动相 ,UV检测波长 3 3 4nm。结果 :浓度在 2 2 .4~ 112 μg·ml-1范围内线性关系良好r =0 .9997,平均回收率 10 0 .7% ,RSD =1.0 % (n =5 )。结论 :该法具有操作简便、结果准确的优点。
OBJECTIVE:To establish an HPLC assay for the determination of rifampicin capsules.METHODS:In the method C 18 column as the stationary phase and acetinitrile- 0.04 mol·L -1 ammonium dihydrogen phosphate (40∶70) as mobile phase the detection wavelength of 334 nm was set.RESULTS:A linearity was obtained from 22.4 ~112 μg·ml -1 of paclitaxel in plasma with a good correlation (r= 0.9997 ). The recovery was 100.7% RSD= 1.0% (n=5).CONCLUSIONS:The method is simple accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2001年第2期86-87,共2页
Chinese Journal of Hospital Pharmacy